MALARIA SPOROZOITE DETECTION BY DISSECTION AND ELISA TO ASSESS INFECTIVITY OF AFROTROPICAL ANOPHELES (DIPTERA, CULICIDAE)
MALARIA SPOROZOITE DETECTION BY DISSECTION AND ELISA TO ASSESS INFECTIVITY OF AFROTROPICAL ANOPHELES (DIPTERA, CULICIDAE)
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DOI：
10.1093/jmedent/27.3.377
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发表时间：
1990-05-01
影响因子：
2.1
通讯作者：
ROBERTS, CR
中科院分区：
文献类型：
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作者：
BEIER, JC;PERKINS, PV;ROBERTS, CR
采用解剖法和恶性疟原虫酶联免疫吸附试验（ELISA）对肯尼亚西部地区20个月采集的26935只冈比亚按蚊和17739只funestus按蚊的疟疾感染率进行比较。ELISA检测的感染率比解剖孢子体的感染率高43%。在解剖阴性按蚊中，10017份唾液腺和237份胸腔样本中分别检测到5.2%和12.2%的环孢子子（CS）蛋白。对352只野外采集的按蚊（保持10 d以确保孢子发育）进行以下试验，比较解剖技术和ELISA技术的准确性：唾液腺解剖、吉氏染色解剖切片检查、唾液腺和胸部身体部位的ELISA试验，以及测定孢子体负荷的显微技术。解剖组、染色玻片组和ELISA组的感染率分别为9.9%、10.8%和15.6%。孢子子负荷与ELISA吸光度值显著相关（r=0.76）。与giemsa染色剥离玻片结果比较，孢子子检测剥离的灵敏度为92.1%，而ELISA检测剥离的灵敏度为78.9%；特别是夹层检测为100.0%，而ELISA检测为92.0%。对非洲热带带菌者头胸标本进行CS蛋白免疫检测，结果显示45.4%的ELISA阳性按蚊不含唾液腺孢子体，因而高估了感染按蚊的比例。
Malaria infection rates determined by dissection and Plasmodium falciparum enzyme-linked immunosorbent assay (ELISA) were compared for 26,935 Anopheles gambiae Giles sensu lato and 17,739 Anopheles funestus Giles collected during 20 mo in western Kenya. ELISA infection rates were about 43% higher than dissection sporozoite rates. In dissection-negative Anopheles, circumsporozoite (CS) protein was detected by ELISA in 5.2% of 10,017 salivary gland samples and in 12.2% of 237 thorax samples. The accuracy of dissection and ELISA techniques was compared by the following tests on a group of 352 field-collected Anopheles (held 10 d to ensure sporogonic development): salivary gland dissection, examination of Giemsa-stained dissection slides, ELISA tests on salivary gland and thorax body parts, and microscopic techniques for determining sporozoite loads. Respective infection rates were 9.9%, 10.8%, and 15.6% for dissection, stained slides, and ELISA. Sporozoite loads were associated significantly with ELISA absorbance values (r=0.76). Compared with Giemsa-stained dissection slide results, the sensitivity of sporozoite detection was 92.1% for dissection compared with 78.9% for ELISA; specifically was 100.0% for dissection versus 92.0% for ELISA. Immunological detection of CS protein in head-thorax samples of Afrotropical vectors overestimated the proportion of infective Anopheles because the comparison of techniques indicated that 45.4% of the ELISA positive Anopheles did not contain salivary gland sporozoites.