High-throughput bacterial functional genomics in the sequencing era.
High-throughput bacterial functional genomics in the sequencing era.
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DOI：
10.1016/j.mib.2015.07.012
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发表时间：
2015-10
影响因子：
5.4
通讯作者：
Gross CA
中科院分区：
文献类型：
--
作者：
Gray AN;Koo BM;Shiver AL;Peters JM;Osadnik H;Gross CA
高通量功能基因组技术正在加速了解细菌生命的多样性和发展对模式细菌生物体的系统水平的理解方面的进展。在这里，我们重点介绍了基于深度测序的功能基因组学的进展，展示了基于全基因组测序的表型分析如何使不愿接受遗传方法的生物体的表型分析成为可能，并叙述了功能基因组方法向非生长表型的快速扩散，并讨论了进展如何使许多不同细菌的基因组规模资源库成为可能。
High-throughput functional genomic technologies are accelerating progress in understanding the diversity of bacterial life and in developing a systems-level understanding of model bacterial organisms. Here we highlight progress in deep-sequencing-based functional genomics, show how phenotyping based on whole genome sequencing is enabling phenotyping in organisms recalcitrant to genetic approaches, and recount the rapid proliferation of functional genomic approaches to non-growth phenotypes, and discuss how advances are enabling genome-scale resource libraries for many different bacteria.