Antibody responses to DNA vaccination of horses using the influenza virus hemagglutinin gene
Antibody responses to DNA vaccination of horses using the influenza virus hemagglutinin gene
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DOI：
10.1016/s0264-410x(98)00496-4
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发表时间：
1999-05-04
期刊：
影响因子：
5.5
通讯作者：
Olsen, CW
中科院分区：
文献类型：
--
作者：
Lunn, DP;Soboll, G;Olsen, CW
马流感病毒感染仍然是马最重要的传染病之一，但目前的疫苗只能提供有限的保护。马对天然流感病毒感染的免疫应答导致长期保护性免疫，其特征在于粘膜伊加和血清IgGa和IgGb抗体应答。DNA疫苗接种提供了传统疫苗的根本替代方案，有可能产生与病毒感染后相同的保护性免疫反应。在大约63天间隔的三次颗粒介导的血凝素（HA）-DNA疫苗接种后，在小马中研究了血清和粘膜分泌物中的抗原特异性抗体同种型反应。一组4匹小马在皮肤和粘膜部位接种疫苗，另一组仅在技能部位接种疫苗。所有的小马在第三次接种后30天进行攻毒感染。皮肤和粘膜接种提供了完全的保护，使其免受感染的临床症状。而皮肤接种提供了部分保护：DNA接种提供了部分保护以防止病毒脱落。DNA疫苗接种仅产生IgGa和IgGb抗体反应，其在皮肤和粘膜接种的小马中以较高的频率发生。没有粘膜IEA反应之前产生的挑战感染和伊加反应，仅检测到在这些小马，摆脱病毒postchallenge。这些结果表明，HA-DNA疫苗接种诱导IgG（a）和IgG（B）抗体应答，这与在不存在粘膜伊加应答的情况下的保护有关。此外，粘膜部位的额外DNA疫苗接种增加了小马的保护和血清转化频率。(C)1999 Elsevier Science Ltd.保留所有权利。
Equine influenza virus infection remains one of the most important infectious diseases of the horse, yet current vaccines offer only limited protection. The equine immune response to natural influenza virus infection results in long-term protective immunity, and is characterized by mucosal IgA and serum IgGa and IgGb antibody responses. DNA vaccination offers a radical alternative to conventional vaccines, with the potential to generate the same protective immune responses seen following viral infection. Antigen-specific antibody isotype responses in serum and mucosal secretions were studied in ponies following particle-mediated delivery of hemagglutinin (HA)-DNA vaccination on three occasions at approximately 63-day intervals. One group of four ponies were vaccinated at skin and mucosal sites and the another group were vaccinated at skill sites only. All ponies were subjected to a challenge infection 30 days after the third vaccination.Skin and mucosal vaccination provided complete protection from clinical signs of infection. while skin vaccination provided partial protection: DNA vaccination provided partial protection from viral shedding. DNA vaccination generated only IgGa and IgGb antibody responses, which occurred with a higher frequency in the skin and mucosa vaccinated ponies. No mucosal IEA response was generated prior to challenge infection and IgA responses were only detected in those ponies which shed virus postchallenge. These results demonstrate that HA-DNA vaccination induces IgG(a) and IgG(b) antibody responses which are associated with protection in the absence of mucosal IgA responses. In addition, additional DNA vaccinations of mucosal sites increased protection and the frequency of seroconversion in ponies. (C) 1999 Elsevier Science Ltd. All rights reserved.