Monolayer culture of primary rat hepatocytes on an Arg-Gly-Asp (RGD)-immobilized polystyrene dish express liver-specific functions of albumin production and p-acetamidophenol metabolism the same as for spheroid culture
Monolayer culture of primary rat hepatocytes on an Arg-Gly-Asp (RGD)-immobilized polystyrene dish express liver-specific functions of albumin production and p-acetamidophenol metabolism the same as for spheroid culture
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DOI：
10.1016/j.bej.2008.01.009
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发表时间：
2008-06-01
影响因子：
3.9
通讯作者：
Kakeya, Yasuo
中科院分区：
文献类型：
--
作者：
Ijima, Hiroyuki;Kakeya, Yasuo
本研究的目的是评估原代大鼠肝细胞的白蛋白产生和药物代谢活性的表达下形成的二维单层培养条件下的RGD-固定化培养皿。在RGD固定的培养皿（2500 ng cm（-2）的Pronectin F包被的培养皿）上的白蛋白产生活性分别是胶原包被的培养皿和球状体培养物的1.6倍（p < 0.05）和1.2倍（p < 0.05）。培养第5天的胶原单层对乙酰氨基苯酚（APAP）代谢率下降至培养第1天活性的16%。另一方面，在7天的培养过程中，RGD-单层和球状体培养物的APAP代谢活性保持良好。然而，我们不能检测到的有效性的RGD单层利多卡因代谢的肝细胞。这是第一份报告表明，肝细胞单层上的RGD-固定化培养基质表达白蛋白的生产和APAP代谢活动等于那些肝细胞球体文化。(C)2008 Elsevier B. V.保留所有权利。
The aim of this study was to evaluate the expression of albumin production and drug metabolism activities of primary rat hepatocytes under a two-dimensional monolayer culture condition formed on a RGD-immobilized culture dish. Albumin production activity on the RGD-immobilized dish (2500 ng cm(-2) Pronectin F-coated dish) was 1.6 (p < 0.05) and 1.2 (p < 0.05) times higher than that on the collagen-coated dish and that of the spheroid culture, respectively. p-Acetamidophenol (APAP) metabolism rate of the collagen-monolayer at 5 days of culture was decreased to 16% of the activity at 1 day of culture. On the other hand, APAP metabolism activities of the RGD-monolayer and spheroid cultures were well maintained during 7 days of culture. However, we could not detect the effectiveness of the RGD-monolayer on lidocaine metabolism of hepatocytes. This is the first report suggesting that a hepatocyte monolayer on a RGD-immobilized culture substratum expresses albumin production and APAP metabolism activities equal to those of a hepatocyte spheroid culture. (C) 2008 Elsevier B.V. All rights reserved.