Lymphocyte blastogenic responses to influenza virus antigens after influenza infection and vaccination in humans
Lymphocyte blastogenic responses to influenza virus antigens after influenza infection and vaccination in humans
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人类感染流感和接种疫苗后淋巴细胞对流感病毒抗原的胚细胞反应
DOI：
10.1128/iai.19.3.867-874.1978
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发表时间：
1978
影响因子：
3.1
通讯作者：
E. A. Caplan
中科院分区：
文献类型：
--
作者：
R. Dolin;B. Murphy;E. A. Caplan
在20名正常志愿者中研究了病毒特异性体外细胞介导的免疫应答，这些志愿者用肝A/维克/3/75（H3 N2）病毒进行攻击，并且在13名志愿者中用含有A/维克和A/NJ/8/76（HswN 1）抗原的灭活疫苗进行接种。从感染或接种疫苗之前和之后不同时间获得的外周血样品建立淋巴细胞培养物。在用纯化的、灭活的完整流感病毒刺激培养物后，通过[3 H]胸苷掺入测定胚细胞生成。感染后6天，显着升高的水平后，观察到在体外刺激与A病毒的血凝素和神经氨酸酶亚型相同（H3 N2）或抗原不同（Heq 1 Neq 1或HswN 1）的挑战病毒，虽然最大的刺激与病毒相同的血凝素亚型（H3）的挑战病毒。在接种后血清抗体升高的接种者的淋巴细胞培养物中观察到类似但更长时间的胚细胞生成反应。这些反应的动力学表明，细胞介导的免疫可能在感染和接种流感病毒后的早期事件中发挥作用。
Virus-specific in vitro cell-mediated immune responses were investigated in 20 normal volunteers who were challenged with liver influenza A/VIC/3/75 (H3N2) virus and in 13 volunteers who were vaccinated with inactivated vaccine containing A/VIC and A/NJ/8/76 (HswN1) antigens. Lymphocyte cultures were established from peripheral blood samples obtained prior to and at various times after infection or vaccination. Blastogenesis was determined by [3H]thymidine incorporation after stimulation of cultures with purified, inactivated, whole influenza viruses. Six days after infection, significantly elevated levels of blastogenesis were observed after in vitro stimulation with A viruses of hemagglutinin and neuraminidase subtypes that were the same as (H3N2) or antigenically distinct from (Heq1Neq1 or HswN1) those of the challenge virus, although maximum stimulation was noted with virus of the same hemagglutinin subtype (H3) as the challenge virus. Similar although more prolonged blastogenic responses were noted in lymphocyte cultures from vaccinees who had serum antibody rises after vaccination. The kinetics of these responses suggest that cell-mediated immunity may play a role in early events after infection and vaccination with influenza virus.