STRUCTURE OF A COMPLEX OF 2 PLASMA-PROTEINS - TRANSTHYRETIN AND RETINOL-BINDING PROTEIN
STRUCTURE OF A COMPLEX OF 2 PLASMA-PROTEINS - TRANSTHYRETIN AND RETINOL-BINDING PROTEIN
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DOI：
10.1126/science.7754382
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发表时间：
1995-05-19
期刊：
影响因子：
56.9
通讯作者：
CODA, A
中科院分区：
文献类型：
--
作者：
MONACO, HL;RIZZI, M;CODA, A
由两种血浆蛋白（运甲状腺素蛋白和视黄醇结合蛋白）形成的复合物的三维结构通过 X 射线衍射数据确定，标称分辨率为 3.1 埃。一个运甲状腺素蛋白四聚体与两个视黄醇结合蛋白分子结合。两个视黄醇结合蛋白分子与相同的转甲状腺素蛋白二聚体建立了分子相互作用，并且每个分子还与其他两个单体之一接触。因此，运甲状腺素蛋白四聚体中的另外两个潜在结合位点被封闭。参与接触的视黄醇结合蛋白的氨基酸残基靠近参与接触的视黄醇结合蛋白的氨基酸残基靠近视黄醇结合位点。
The three-dimensional structure of the complex formed by two plasma proteins, transthyretin and retinol-binding protein, was determined from x-ray diffraction data to a nominal resolution of 3.1 angstroms. One tetramer of transthyretin was bound to two molecules of retinol-binding protein. The two retinol-binding protein molecules established molecular interactions with the same transthyretin dimer, and each also made contacts with one of the other two monomers. Thus, the other two potential binding sites in a transthyretin tetramer were blocked. The amino acid residues of the retinol-binding protein that were involved in the contacts were close to the retinol-binding protein that were involved in the contacts were close to the retinol-binding site.