Transcriptional expression of Stilbene synthase genes are regulated developmentally and differentially in response to powdery mildew in Norton and Cabernet Sauvignon grapevine
Transcriptional expression of Stilbene synthase genes are regulated developmentally and differentially in response to powdery mildew in Norton and Cabernet Sauvignon grapevine
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DOI：
10.1016/j.plantsci.2012.09.004
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发表时间：
2012-12-01
期刊：
影响因子：
5.2
通讯作者：
Qiu, Wenping
中科院分区：
文献类型：
--
作者：
Dai, Ru;Ge, Hui;Qiu, Wenping
苯乙烯类化合物是天然植物抗毒素，在植物防御病原体中具有抗菌活性。二苯乙烯合成酶（STS）是催化二苯乙烯类化合物生物合成的关键酶。葡萄基因组包含35个初步注释的STS基因家族，每个STS基因的调控需要研究以确定其作用。本研究选取了8个STS基因，分别为STS8、STS27/31、STS16/22、STS13/17/23，应用定量聚合酶链反应（qPCR）分析了它们在白粉病真菌（PM）、Elysiphe necator （Schw.）侵染后叶片组织中的转录表达谱。毛刺。他们的转录本也在葡萄的幼叶和老叶以及葡萄的五个发育阶段的浆果皮中进行了比较，葡萄的赤霞珠和葡萄的诺顿。结果表明，接种PM孢子后24和48 h，赤霞珠叶片中选定的STS基因转录本显著增加，而诺顿叶片对PM侵染的反应基本不变。与赤霞珠相比，诺顿老叶中STS8、STS27/31和STS13/17/23转录本的含量更高。STS基因在幼叶中的表达量低于老叶。赤霞珠和诺顿的果实皮中8个STS基因的转录水平急剧增加。此外，草莓果皮中反式白藜芦醇含量在改良后迅速增加，在收获时达到最高水平。这些试验表明，在两个葡萄品种中，单个STS基因在对PM感染的反应和发育过程中受到不同的调控。本研究为进一步研究葡萄中各STS基因的调控和功能提供了基础知识，并为葡萄基因组中STS基因家族的功能注释提供了实验依据。2012爱思唯尔爱尔兰有限公司版权所有。
Stilbenic compounds are natural phytoalexins that have antimicrobial activities in plant defense against pathogens. Stilbene synthase (STS) is the key enzyme that catalyzes the biosynthesis of stilbenic compounds. Grapevine genome contains a family of preliminarily annotated 35 STS genes, the regulation of each STS gene needs to be studied to define their roles. In this study, we selected eight STS genes, STS8, STS27/31,STS16/22,STS13/17/23, and applied quantitative polymerase chain reaction (qPCR) to characterize their transcriptional expression profiles in leaf tissues upon infection by the powdery mildew fungus (PM), Elysiphe necator (Schw.) Burr. Their transcripts were also compared in young and old leaves as well as in the berry skin at five developmental stages in Vitis vinifera 'Cabernet Sauvignon' and Vitis aestivalis 'Norton'. The results showed that transcripts of selected STS genes increased significantly in Cabernet Sauvignon leaves at 24 and 48 h post inoculation with PM spores and remained unchanged in Norton leaves in response to the PM infection. Transcripts of STS8, STS27/31 and STS13/17/23 were more abundant in the old leaves of Norton than in Cabernet Sauvignon. STS genes showed lower expression levels in young leaves than in old leaves. Transcript levels of the eight STS genes increased drastically in the berry skin of Cabernet Sauvignon and Norton post veraison. In addition, the content of trans-resveratrol in the berry skin rapidly increased post veraison and reached the highest level at harvest. These assays demonstrated that individual STS genes are regulated differentially in response to PM infection and during development in the two grape varieties. The present study yields basic knowledge for further investigation of the regulation and function of each STS gene in grapevine and provides experimental evidences for the functional annotation of the STS gene family in the grapevine genome. (C) 2012 Elsevier Ireland Ltd. All rights reserved.