Apigenin inhibited migration and invasion of human ovarian cancer A2780 cells through focal adhesion kinase
Apigenin inhibited migration and invasion of human ovarian cancer A2780 cells through focal adhesion kinase
复制标题
芹菜素通过粘着斑激酶抑制人卵巢癌A2780细胞的迁移和侵袭
DOI：
10.1093/carcin/bgn244
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发表时间：
2008-12-01
期刊：
影响因子：
4.7
通讯作者：
Fang, Jing
中科院分区：
文献类型：
--
作者：
Hu, Xiao-Wen;Meng, Dan;Fang, Jing
芹菜素是一种常见的膳食黄酮类化合物，已被发现具有抗肿瘤特性，因此对癌症化学预防和/或化疗剂的开发产生了特别的兴趣。在这里，我们证明芹菜素抑制粘着斑激酶（FAK）的表达以及人卵巢癌A2780细胞的迁移和侵袭。 FAK 是整合素和生长因子下游的非受体蛋白酪氨酸激酶。它在癌细胞的迁移和侵袭中发挥重要作用。我们发现芹菜素抑制A2780细胞的粘附、迁移和侵袭。芹菜素通过降低其蛋白质稳定性来减弱 FAK 的表达。 FAK 在 A2780 细胞的迁移和侵袭中发挥着关键作用。 FAK的过表达可以逆转芹菜素抑制的A2780细胞迁移和侵袭。体内实验表明芹菜素可抑制裸鼠卵巢A2780细胞的自发转移。我们的结果为芹菜素抑制卵巢癌的机制提供了新的见解。这些结果表明芹菜素对 FAK 的分子靶向可能是卵巢癌化学预防和/或化疗的有用策略。
Apigenin, a common dietary flavonoid, has been found to have antitumor properties and therefore poses special interest for the development of chemopreventive and/or chemotherapeutic agent for cancers. Here, we demonstrate that apigenin inhibits expression of focal adhesion kinase (FAK) and migration and invasion of human ovarian cancer A2780 cells. FAK is a non-receptor protein tyrosine kinase downstream of integrins and growth factors. It plays an important role in migration and invasion of cancer cells. We found that apigenin inhibited adhesion, migration and invasion of A2780 cells. Apigenin attenuated FAK expression through reducing its protein stability. FAK plays a critical role in migration and invasion of A2780 cells. Overexpression of FAK could reverse A2780 cell migration and invasion inhibited by apigenin. The in vivo experiments showed that apigenin inhibited spontaneous metastasis of A2780 cells implanted onto the ovary of nude mice. Our results provide a new insight into the mechanisms that apigenin inhibits ovarian cancers. These results suggest that molecular targeting of FAK by apigenin might be a useful strategy for chemoprevention and/or chemotherapeutics of ovarian cancers.