Administration of Gonadotropins for Superovulation in Mice.
Administration of Gonadotropins for Superovulation in Mice.
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DOI：
10.1101/pdb.prot092403
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发表时间：
2018-01-02
影响因子：
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通讯作者：
Nagy, Andras
中科院分区：
文献类型：
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作者：
Behringer, Richard;Gertsenstein, Marina;Nagy, Andras
对于需要大量植入前胚胎的实验，如显微注射受精卵，在交配前向雌性动物注射促性腺激素，以增加排卵的卵母细胞数量(即诱导超数排卵)。用孕马血清促性腺激素(PMSG)模拟内源性卵泡刺激素(FSH)的卵母细胞成熟效应，用人绒毛膜促性腺激素(HCG)模拟黄体生成素(LH)的促排卵作用。
For experiments that require large numbers of preimplantation mouse embryos, such as microinjection of zygotes, gonadotropins are administered to females before mating to increase the number of oocytes that are ovulated (i.e., to induce superovulation). Pregnant mare serum gonadotropin (PMSG) is used to mimic the oocyte maturation effect of the endogenous follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG) is used to mimic the ovulation induction effect of luteinizing hormone (LH).