Novel proteomic approaches for tissue analysis
Novel proteomic approaches for tissue analysis
复制标题
用于组织分析的新型蛋白质组学方法
DOI：
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
R. Chuaqui
中科院分区：
文献类型：
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作者：
M. Tangrea;Benjamin S. Wallis;J. Gillespie;G. Gannot;M. Emmert;R. Chuaqui
蛋白质组学是对蛋白质表达和特征的全球性研究，近年来已成为分子分析领域的一个重要组成部分。蛋白质的动态性质，从离子通道到分子伴侣，提出了一个挑战，但对这些分子的理解提供了丰富的信息来源。当将蛋白质组学分析直接应用于人体组织样本时，会出现额外的困难。以下文章概述了目前用于组织分析的蛋白质组学工具，从常规方法如蛋白质印迹分析和2D聚丙烯酰胺凝胶电泳开始。最新的高通量技术正在使用的今天也进行了审查。这些包括蛋白质阵列、反相蛋白质裂解物阵列、基质辅助激光解吸/电离、表面增强激光解吸/电离和分层表达扫描。此外，还包括生物信息学以及有关组织保存和显微切割以获得纯细胞群的问题。最后，对组织蛋白质组学的发展方向进行了展望。
Proteomics, the global study of protein expression and characteristics, has recently emerged as a key component in the field of molecular analysis. The dynamic nature of proteins, from ion channels to chaperones, presents a challenge, yet the understanding of these molecules provides a rich source of information. When applying proteomic analysis directly to human tissue samples, additional difficulties arise. The following article presents an overview of the current proteomic tools used in the analysis of tissues, beginning with conventional methods such as western blot analysis and 2D polyacrylamide gel electrophoresis. The most current high-throughput techniques being used today are also reviewed. These include protein arrays, reverse-phase protein lysate arrays, matrix-assisted laser desorption/ionization, surface-enhanced laser desorption/ionization and layered expression scanning. In addition, bioinformatics as well as issues regarding tissue preservation and microdissection to obtain pure cell populations are included. Finally, future directions of the tissue proteomics field are discussed.
DOI：
10.1073/pnas.88.2.340
发表时间：
1991-01-01
影响因子：
11.1
作者：
SMEEKENS, SP;AVRUCH, AS;STEINER, DF
通讯作者：
STEINER, DF
小鼠激素原转化酶 1 前结构域的稳定性和全局折叠。
影响因子：
2.9
作者：
Tangrea,MA;Alexander,P;Bryan,PN;Eisenstein,E;Toedt,J;Orban,J
通讯作者：
Orban,J