Transformation of nematodes via ballistic DNA transfer

Transformation of nematodes via ballistic DNA transfer
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DOI:
10.1016/s0166-6851(99)00089-4
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发表时间:
1999-10-15
影响因子:
1.5
通讯作者:
Hobom, G
Hobom, G
中科院分区:
医学4区
文献类型:
--
作者:
Jackstadt, P;Wilm, TP;Hobom, G

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描述了一种利用金颗粒进行弹道DNA转移转染秀丽隐杆线虫和sigmodonislitomosoides的方法。质粒DNA分子被证明通过扩散分布在蠕虫的整个身体,并有可能转染所有体细胞。
A method is described for the transfection ofCaenorhabditis elegansandLitomosoides sigmodontisby ballistic DNA transfer employing gold particles. The plasmid DNA molecules were shown to be distributed by diffusion throughout the entire body of the worm and to have the potential to transfect all somatic cells.