GENETIC IMMUNIZATION IS A SIMPLE METHOD FOR ELICITING AN IMMUNE-RESPONSE
GENETIC IMMUNIZATION IS A SIMPLE METHOD FOR ELICITING AN IMMUNE-RESPONSE
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DOI：
10.1038/356152a0
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发表时间：
1992-03-12
期刊：
影响因子：
64.8
通讯作者：
JOHNSTON, SA
中科院分区：
文献类型：
--
作者：
TANG, DC;DEVIT, M;JOHNSTON, SA
为了产生针对外来蛋白质的免疫反应，通常需要纯化该蛋白质，然后将其注射到动物体内。分离足够的纯蛋白质非常耗时，有时甚至很困难。在这里，我们报告说，通过将编码蛋白质的基因直接引入小鼠皮肤也可以引发这种反应。这是通过使用手持式生物射弹系统 1-4 来实现的，该系统可以将 DNA 包被的金微弹直接推进到活体动物的细胞中 3,5,6。基因免疫在产生抗体方面可以节省时间和劳力，并且可以提供独特的疫苗接种方法。
To produce an immune reaction against a foreign protein usually requires purification of that protein, which is then injected into an animal. The isolation of enough pure protein is time-consuming and sometimes difficult. Here we report that such a response can also be elicited by introducing the gene encoding a protein directly into the skin of mice. This is achieved using a hand-held form of the biolistic system 1-4 which can propel DNA-coated gold microprojectiles directly into cells in the living animal 3,5,6. Genetic immunization may be time- and labour-saving in producing antibodies and may offer a unique method for vaccination.