Differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets.
Differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets.
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发表时间：
1986-12
期刊：
影响因子：
11.2
通讯作者：
W. Rettig;P. G. Chesa;H. Beresford;Hans-Joachim Feickert;Mark T Jennings;J. Cohen;Herbert F. Oettgen;L. Old;H-J F
中科院分区：
文献类型：
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作者：
W. Rettig;P. G. Chesa;H. Beresford;Hans-Joachim Feickert;Mark T Jennings;J. Cohen;Herbert F. Oettgen;L. Old;H-J F
我们的特点是五个不同的细胞表面抗原的人星形细胞瘤和相关的表达与胶质细胞酸性蛋白（GFAP）和四个以前定义的细胞表面标志物的星形细胞瘤的表达。新研究的抗原之一A4最初在大鼠中枢神经系统（但不是外周神经系统）神经元上检测到，其在GFAP+人星形细胞瘤细胞上表达，但不在GFAP-星形细胞瘤或广泛的其他神经外胚层、上皮和造血细胞上表达。抗原F19（Mr 140，000/90，000糖蛋白）和F24（Mr 90，000糖蛋白）也显示出有限的分布，并在神经外胚层和间充质细胞的亚群上表达。抗原G253（Mr 95，000糖蛋白）和S5（Mr 120，000糖蛋白）在培养细胞组中分布更广。在一系列22个星形细胞瘤细胞系和正常脑组织中测定这些抗原的分布，并将结果与5种其他胶质细胞标志物的分布进行比较：GFAP和细胞表面抗原A010（Mr 110，000糖蛋白）; AJ 8（Mr 100，000糖蛋白）; LK 26（Mr 35，000糖蛋白）;和Thy-1。不同的模式，培养的星形细胞瘤和神经组织中的表达被确定为所有的抗原系统研究，和细胞表面表达的抗原A4被发现与GFAP表型培养的星形细胞瘤密切相关。本研究中描述的抗原为研究正常胶质细胞分化和星形细胞瘤不同亚群的表型和生物学行为相关性提供了新的标志物。
We have characterized five distinct cell surface antigens of human astrocytomas and correlated their expression with the expression of glial fibrillary acidic protein (GFAP) and four previously defined cell surface markers of astrocytomas. One of the newly studied antigens, A4, which was originally detected on rat central nervous system (but not peripheral nervous system) neurons, is expressed on GFAP+ human astrocytoma cells, but not on GFAP- astrocytomas or a wide range of other neuroectodermal, epithelial, and hematopoietic cells. Antigens F19 (Mr 140,000/90,000 glycoprotein) and F24 (Mr 90,000 glycoprotein) also show restricted distribution and are expressed on subsets of neuroectodermal and mesenchymal cells. Antigens G253 (Mr 95,000 glycoprotein) and S5 (Mr 120,000 glycoprotein) are more widely distributed on the cultured cell panel. The distribution of these antigens was determined on a series of 22 astrocytoma cell lines and in normal brain tissue and the results were compared with the distribution of 5 additional glial cell markers: GFAP and cell surface antigens A010 (Mr 110,000 glycoprotein); AJ8 (Mr 100,000 glycoprotein); LK26 (Mr 35,000 glycoprotein); and Thy-1. Distinct patterns of expression on cultured astrocytomas and in neural tissues were identified for all antigenic systems studied, and cell surface expression of antigen A4 was found to correlate closely with GFAP phenotype of cultured astrocytomas. The antigens described in this study provide new markers to study normal glial differentiation and to correlate the phenotypes and biological behavior of distinct subsets of astrocytomas.