Information-theoretical analysis of time-correlated single-photon counting measurements of single molecules.
Information-theoretical analysis of time-correlated single-photon counting measurements of single molecules.
复制标题
单分子时间相关单光子计数测量的信息理论分析。
DOI：
10.1021/jp8082908
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Talaga,DavidS
中科院分区：
文献类型：
--
作者：
Talaga,DavidS
时间相关的单光子计数允许在单分子水平上确定发光寿命信息。本文发展了一种形式主义，使信息论分析的能力，发光寿命测量，以解决在一个单一的分子状态。它分析了光子流的信息内容以及与状态确定问题相关的信息部分。实验损失的信息，由于仪器的响应，数字化，和不同类型的背景进行计算和程序，以确定实验参数的最佳值。本文展示了如何使用信息理论的形式主义来评估所需的光子数来区分染料，不同的只是寿命。它扩展了这一想法，包括区分不同的电子转移猝灭或共振能量转移的分子状态，并显示如何信号和背景的寿命之间的差异可以帮助区分染料的位置在激发光束。
Time-correlated single photon counting allows luminescence lifetime information to be determined on a single molecule level. This paper develops a formalism to allow information theory analysis of the ability of luminescence lifetime measurements to resolve states in a single molecule. It analyzes the information content of the photon stream and the fraction of that information that is relevant to the state determination problem. Experimental losses of information due to instrument response, digitization, and different types of background are calculated and a procedure to determine the optimal value of experimental parameters is demonstrated. This paper shows how to use the information theoretical formalism to evaluate the number of photons required to distinguish dyes that differ only by lifetime. It extends this idea to include distinguishing molecular states that differ in the electron transfer quenching or resonant energy transfer and shows how the differences between the lifetime of signal and background can help distinguish the dye position in an excitation beam.
单分子
DOI：
10.1073/pnas.191365898
发表时间：
2001
影响因子：
11.1
作者：
Th. Basché;S. Nie;J. M. Fernandez
通讯作者：
J. M. Fernandez