ISOLATION AND STRUCTURAL CHARACTERIZATION OF INSULIN AND GLUCAGON FROM THE HOLOCEPHALAN SPECIES CALLORHYNCHUS-MILII (ELEPHANTFISH)
ISOLATION AND STRUCTURAL CHARACTERIZATION OF INSULIN AND GLUCAGON FROM THE HOLOCEPHALAN SPECIES CALLORHYNCHUS-MILII (ELEPHANTFISH)
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DOI：
10.1042/bj2630261
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发表时间：
1989-10-01
影响因子：
4.1
通讯作者：
CUTFIELD, JF
中科院分区：
文献类型：
--
作者：
BERKS, BC;MARSHALL, CJ;CUTFIELD, JF
来自全头动物软骨鱼 Callorhynchus milii（象鱼）胰腺的胰岛素和胰高血糖素已被分离和纯化。两台反相高效液相色谱仪这些步骤能够回收足够的材料用于完整链以及肽消化产物的气相测序。象鱼胰岛素序列显示与猪胰岛素有 14 处差异，其中包括两个不寻常的替代。胰岛素 B 链含有 31 个残基，比哺乳动物胰岛素多 1 个，但明显少于密切相关的大鼠鱼，否则它与大鼠表现出高度的序列相似性。象鱼和猪胰高血糖素仅在四个位置上有所不同，但与大鼠鱼胰高血糖素 36（正常胰高血糖素包含 29 个残基）序列相比，有 6 个变化。显然，不同的激素原蛋白水解加工机制在两种全头藻物种中发挥着作用。
Both insulin and glucagon from the pancreas of the holocephalan cartilaginous fish Callorhynchus milii (elephantfish) have been isolated and purified. Two reverse-phase h.p.l.c. steps enabled recovery of sufficient material for gas-phase sequencing of the intact chains as well as peptide digestion products. The elephantfish insulin sequence shows 14 differences from pig insulin, including two unusual substitutions. The insulin B-chain contains 31 residues, one more than mammalian insulins, but markedly less than that of the closely related ratfish with which it otherwise exhibits high sequence similarity. Elephantfish and pig glucagons differ at only four positions, but there are six changes from the ratfish glucagon-36 (normal glucagon contains 29 residues) sequence. It is apparent that different prohormone proteolytic processing mechanisms operate in the two holocephalan species.