A custom-built low-cost chamber for exposing rodents to e-cigarette aerosol: practical considerations
A custom-built low-cost chamber for exposing rodents to e-cigarette aerosol: practical considerations
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DOI：
10.1080/08958378.2019.1698678
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发表时间：
2019-12-03
影响因子：
2.1
通讯作者：
Kleiman, Norman J.
中科院分区：
文献类型：
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作者：
Hilpert, Markus;Ilievski, Vesna;Kleiman, Norman J.
目标：(1) 设计和构建低成本暴露室系统，使啮齿动物全身暴露于电子烟气溶胶，(2) 提供详细说明，特别关注自动电子烟激活，以及 (3) 开发暴露室中气溶胶水平的简单数学模型。方法：我们使用标准实验室设备和用于电子烟激活的开源电子平台（Arduino）构建了系统。 Arduino 用于控制螺线管，该螺线管按下所谓的“Mod”电子烟的激活按钮，以及用于将气溶胶从电子烟烟嘴移入腔室的泵。对于“Pods”和“Cigalikes”，不使用电磁阀，因为它们是由泵产生的真空激活的。用光散射激光光度计测量气溶胶浓度。结果：该系统允许改变空气交换率，监测气溶胶水平，并对任意抽吸地形进行编程。不同室操作条件（抽吸时间和周期、电子烟功率输出、空气交换率）观察到的气溶胶浓度与数学模型一致。结论：我们的低成本暴露室可用于电子烟对健康影响的动物实验研究。我们的模型可以估计设计参数，例如腔室容积、空气交换率和抽吸时间。
Objectives: To (1) design and build a low-cost exposure chamber system for whole-body exposure of rodents to electronic cigarette aerosol, (2) provide detailed instructions with particular focus on automated e-cigarette activation, and (3) develop a simple mathematical model for aerosol levels in the exposure chamber. Methods: We built the system with standard laboratory equipment and an open-source electronics platform (Arduino) for e-cigarette activation. Arduino is used to control a solenoid, which pushes the activation button of so-called "Mod" e-cigarettes, and a pump to move the aerosol from the mouthpiece of the e-cigarette into the chamber. For "Pods" and "Cigalikes," the solenoid is not used as they are activated by the vacuum created by the pump. Aerosol concentrations were measured with a light-scattering laser photometer. Results: The system allows varying the air exchange rate, monitoring aerosol levels, and programing arbitrary puff topography. Aerosol concentrations observed for different chamber operating conditions (puff time and period, e-cigarette power output, air exchange rate) were consistent with the mathematical model. Conclusions: Our low-cost exposure chamber can be used in animal experimental studies of the health effects of e-cigarettes. Our model allows estimating design parameters such as chamber volume, air exchange rate, and puff period.