CXCL12 is expressed by skeletal muscle cells in tongue oral squamous cell carcinoma.
CXCL12 is expressed by skeletal muscle cells in tongue oral squamous cell carcinoma.
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CXCL12由舌头口服鳞状细胞癌中的骨骼肌细胞表达。
作者：
CXCL12/CXCR4轴在包括口腔鳞状细胞癌（OSCC）在内的各种恶性肿瘤的进展中发挥着关键作用。在本研究中，我们旨在阐明CXCL12在OSCC肿瘤微环境中的生物学和临床意义。使用公开的单细胞 RNA 测序 (RNA-seq) 数据集来分析头颈鳞状细胞癌 (HNSCC) 中的 CXCL12 表达。对 47 个手术切除的原发性舌 OSCC 进行了 CXCL12、α-平滑肌抗原 (α-SMA)、成纤维细胞激活蛋白 (FAP) 和 CD8 的免疫组织化学分析。将人骨骼肌细胞与或不与 OSCC 细胞共培养，然后使用定量逆转录 PCR 分析 CXCL12 表达。 RNA-seq 数据分析表明 CXCL12 在 HNSCC 组织内的基质细胞中大量表达。免疫组织化学分析显示，在 1 级原发性 OSCC 中，CXCL12 在肿瘤细胞和肌肉细胞中表达。相比之下，3 级肿瘤的特点是肌肉结构破坏和 CXCL12 表达减少。对肿瘤内 CXCL12 阳性区域的定量分析表明，CXCL12 表达减少与总体生存率较差相关。 CXCL12 表达水平往往与 α-SMA 表达呈负相关，与 CD8+ 淋巴细胞浸润呈正相关，但这些关系并未达到统计学显着性。 CXCL12 在与 OSCC 细胞共培养的肌肉细胞中显着上调。我们的结果表明舌 OSCC 细胞激活肌肉细胞中的 CXCL12 表达，这可能有助于肿瘤进展。然而，CXCL12 在晚期 OSCC 中由于肌肉组织破坏而减少。我们的结果表明舌 OSCC 细胞激活肌肉细胞中的 CXCL12 表达，这可能有助于肿瘤进展。然而，CXCL12 在晚期 OSCC 中由于肌肉组织破坏而减少。
The CXCL12/CXCR4 axis plays a pivotal role in the progression of various malignancies, including oral squamous cell carcinoma (OSCC). In this study, we aimed to clarify the biological and clinical significance of CXCL12 in the tumor microenvironment of OSCCs. Publicly available single‐cell RNA‐sequencing (RNA‐seq) datasets were used to analyze CXCL12 expression in head and neck squamous cell carcinomas (HNSCC). Immunohistochemical analysis of CXCL12, α‐smooth muscle antigen (α‐SMA), fibroblast activation protein (FAP) and CD8 was performed in a series of 47 surgically resected primary tongue OSCCs. Human skeletal muscle cells were co‐cultured with or without OSCC cells, after which CXCL12 expression was analyzed using quantitative reverse‐transcription PCR. Analysis of the RNA‐seq data suggested CXCL12 is abundantly expressed in stromal cells within HNSCC tissue. Immunohistochemical analysis showed that in grade 1 primary OSCCs, CXCL12 is expressed in both tumor cells and muscle cells. By contrast, grade 3 tumors were characterized by disruption of muscle structure and reduced CXCL12 expression. Quantitative analysis of CXCL12‐positive areas within tumors revealed that reduced CXCL12 expression correlated with poorer overall survival. Levels of CXCL12 expression tended to inversely correlate α‐SMA expression and positively correlate with infiltration by CD8+ lymphocytes, though these relations did not reach statistical significance. CXCL12 was significantly upregulated in muscle cells co‐cultured with OSCC cells. Our results suggest that tongue OSCC cells activate CXCL12 expression in muscle cells, which may contribute to tumor progression. However, CXCL12 is reduced in advanced OSCCs due to muscle tissue destruction. Our results suggest that tongue OSCC cells activate CXCL12 expression in muscle cells, which may contribute to tumor progression. However, CXCL12 is reduced in advanced OSCCs due to muscle tissue destruction.
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影响因子：
29.4
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Garg B;Giri B;Modi S;Sethi V;Castro I;Umland O;Ban Y;Lavania S;Dawra R;Banerjee S;Vickers S;Merchant NB;Chen SX;Gilboa E;Ramakrishnan S;Saluja A;Dudeja V
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影响因子：
4.7
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4.8
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影响因子：
50.3
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通讯作者：
Mechta-Grigoriou, Fatima
影响因子：
5.7
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