Membrane proximal lysosomes are the major vesicles responsible for calcium-dependent exocytosis in nonsecretory cells.
Membrane proximal lysosomes are the major vesicles responsible for calcium-dependent exocytosis in nonsecretory cells.
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膜近端溶酶体是负责非分泌细胞中钙依赖性胞吐作用的主要囊泡。
DOI：
10.1083/jcb.200208154
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发表时间：
2002-11-25
影响因子：
7.8
通讯作者：
Simon, Sanford M
中科院分区：
文献类型：
--
作者：
Jaiswal, Jyoti K;Andrews, Norma W;Simon, Sanford M
与钙在分泌细胞中的作用类似，钙可以在非分泌细胞中触发胞吐作用。这种钙依赖的胞吐作用对于修复膜破裂是必不可少的。利用全内反射荧光显微镜，我们观察到参与这一过程的许多细胞器，包括内质网、高尔基体后小泡、晚期内小体、早期内小体和溶酶体，都位于质膜的100 nm以内(在消失场中)。然而，细胞内钙的增加只导致溶酶体的胞吐作用。融合的溶酶体主要停靠在质膜上，表明钙是融合的主要原因，而不是溶酶体募集到细胞表面。
Similar to its role in secretory cells, calcium triggers exocytosis in nonsecretory cells. This calcium-dependent exocytosis is essential for repair of membrane ruptures. Using total internal reflection fluorescence microscopy, we observed that many organelles implicated in this process, including ER, post-Golgi vesicles, late endosomes, early endosomes, and lysosomes, were within 100 nm of the plasma membrane (in the evanescent field). However, an increase in cytosolic calcium led to exocytosis of only the lysosomes. The lysosomes that fused were predominantly predocked at the plasma membrane, indicating that calcium is primarily responsible for fusion and not recruitment of lysosomes to the cell surface.