Embryonic dermal condensation and adult dermal papilla induce hair follicles in adult glabrous epidermis through different mechanisms
Embryonic dermal condensation and adult dermal papilla induce hair follicles in adult glabrous epidermis through different mechanisms
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DOI：
10.1111/j.1440-169x.2006.00848.x
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发表时间：
2006-02-01
影响因子：
2.5
通讯作者：
Yoshizato, K
中科院分区：
文献类型：
--
作者：
Inamatsu, M;Tochio, T;Yoshizato, K
比较了胚胎真皮和成人真皮对成人无毛表皮的毛发诱导作用。将由成人足底表皮和含真皮浓缩物（DC）的胚胎真皮组成的重组皮肤移植到裸鼠背部。移植物的表皮形成毛。诱导过程的组织学证明了板状组织的形成，表明移植物通过与胚胎皮肤中毛囊发育的机制相似的机制产生毛囊。一个单独的成年大鼠唯一的皮肤片，插入与培养的毛乳头（DP）细胞的聚集体或完整的DP之间的表皮和真皮，类似地移植。移植产生了毛囊。组织学显示，在这两种情况下，表皮包围DP细胞的聚集。表皮从未形成基板样组织。因此，可以得出结论，当暴露于DC时，成年表皮细胞重演毛囊发育的胚胎过程，而当暴露于DP时，它们直接进入毛发周期的生长期。Edar和Shh基因以及P-cadherin蛋白在两种移植物毛囊发育过程中的表达模式支持上述结论。
Hair induction in the adult glabrous epidermis by the embryonic dermis was compared with that by the adult dermis. Recombinant skin, composed of the adult sole epidermis and the embryonic dermis containing dermal condensations (DC), was transplanted onto the back of nude mice. The epidermis of transplants formed hairs. Histology on the induction process demonstrated the formation of placode-like tissues, indicating that the transplant produces hair follicles through a mechanism similar to that underlying hair follicle development in the embryonic skin. An isolated adult rat sole skin piece, inserted with either an aggregate of cultured dermal papilla (DP) cells or an intact DP between its epidermis and dermis, was similarly transplanted. The transplant produced hair follicles. Histology showed that the epidermis in both cases surrounded the aggregates of DP cells. The epidermis never formed placode-like tissues. Thus, it was concluded that the adult epidermal cells recapitulate the embryonic process of hair follicle development when exposed to DC, whereas they get directly into the anagen of the hair cycle when exposed to DP. The expression pattern of Edar and Shh genes, and P-cadherin protein during the hair follicle development in the two types of transplants supported the above conclusion.