Subtypes of active cell death in the granulosa of ovarian atretic follicles in the quail (Coturnix coturnix japonica)
Subtypes of active cell death in the granulosa of ovarian atretic follicles in the quail (Coturnix coturnix japonica)
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DOI：
10.1051/rnd:19960203
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发表时间：
1996-01-01
期刊：
影响因子：
--
通讯作者：
Roels, F
中科院分区：
文献类型：
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作者：
DHerde, K;DePrest, B;Roels, F
应用细胞化学和电镜技术对日本鹌鹑卵巢卵泡闭锁进行了研究。三种不同类型的细胞死亡共存的颗粒。大量细胞出现凋亡迹象。这些细胞中的DNA片段化在先前的研究中使用原位末端标记证明。第二种不可忽略的细胞死亡类型包括细胞质的广泛自噬作用，同时发生晚期核改变。最后，少数分离的细胞显示细胞质崩解和染色质凝聚的小的不规则团块，指示原发性细胞坏死。细胞凋亡与自噬细胞死亡揭示了不同的酸性磷酸酶活性模式（溶酶体与细胞质）。我们建议，这些意见可能与存在不同的亚群的颗粒已被其他人所示。这项研究证实了颗粒细胞死亡的生化数据，但表明细胞凋亡不是卵泡闭锁中活跃细胞死亡的唯一模式。
Follicular atresia in the ovaries of Japanese quail was studied by cytochemistry and electron microscopy. Three different types of cell death coexisted in the granulosa. A large number of cells showed signs of apoptosis. The DNA fragmentation in these cells was demonstrated in a previous study using in situ end-labeling. A second and non-negligible type of cell death consisted of extensive autophagocytosis of the cytoplasm occurring simultaneously with late nuclear alterations. Finally, a few detached cells displayed cytoplasmic disintegration and small irregular clumps of chromatin condensation indicative of primary cell necrosis. Apoptotic versus autophagic cell death revealed a different pattern of acid phosphatase activity (lysosomal versus cytoplasmic). We propose that these observations may be linked to the existence of distinct subpopulations in the granulosa as has been shown by others. This study confirms the biochemical data on granulosa cell death, but demonstrates that apoptosis is not the exclusive mode of active cell death in follicular atresia.