Protein resistance of surfaces prepared by chemisorption of monothiolated poly(ethylene glycol) to gold and dendronization with aliphatic polyester dendrons: Effect of hydrophilic dendrons
Protein resistance of surfaces prepared by chemisorption of monothiolated poly(ethylene glycol) to gold and dendronization with aliphatic polyester dendrons: Effect of hydrophilic dendrons
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DOI：
10.1021/ma702074v
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发表时间：
2008-04-08
期刊：
影响因子：
5.5
通讯作者：
Adronov, Alex
中科院分区：
文献类型：
--
作者：
Benhabbour, S. Rahima;Liu, Lina;Adronov, Alex
通过将硫醇封端的聚乙二醇 (HS-PEG(650)-OH) 化学吸附到 g-old 涂覆的硅片上，然后用脂肪族聚酯树枝对末端 PEG OH 基团进行官能化，研究了蛋白质对表面的吸附。 FIS-PEG(650)-OH 在浊点条件下对金表面进行化学吸附，得到类似于 3.7 chams/nm(2) 的链密度（根据 AFM 膜厚度测量计算得出）。使用发散树突生长实现了第 1-4 代脂肪族聚酯树突的 PEG 功能化表面的树突化。如水接触角数据所示，表面的亲水性随着树枝化生成的增加而显着增加。报道了亲水性树突对磷酸盐缓冲盐水 (PBS) 和血浆中蛋白质吸附的影响。 I-125-放射性纤维蛋白原和溶菌酶在树枝化表面上的吸附表明，在将树枝引入 PEG 功能化表面后，蛋白质吸附增加。纤维蛋白原和溶菌酶吸附之间的相似性表明，无论蛋白质大小如何，树枝状表面对蛋白质的抵抗力都遵循相同的趋势。
Protein adsorption to surfaces prepared by chemisorption of thiol-terminated poly(ethylene glycol) (HS-PEG(650)-OH) to g-old-coated silicon wafers followed by functionalization of the terminal PEG OH groups with aliphatic polyester dendrons was investigated. Chemisorption of FIS-PEG(650)-OH to the gold surfaces was carried Out Under cloud-point conditions to give a chain density of similar to 3.7 chams/nm(2), as calculated from AFM film thickness measurements. Dendronization of the PEG-functionalized surfaces with aliphatic polyester dendrons, generations 1-4, was achieved using divergent dendron growth. The hydrophilicity of the surfaces increased significantly with increasing dendron generation as shown by water contact angle data. The effect of the hydrophilic dendrons on protein adsorption from phosphate-buffered saline (PBS) and plasma are reported. Adsorption of both I-125-radiolabled fibrinogen and lysozyme onto the dendronized surfaces showed that protein adsorption increases upon introduction of dendrons to the PEG-functionalized Surfaces. The similarity between fibrinogen and lysozyme adsorption suggests that resistance of the dendronized Surfaces to proteins follows the same trend regardless of protein size.