Differentiating nicotine- versus schizophrenia-associated decreases of the α7 nicotinic acetylcholine receptor transcript, CHRFAM7A, in peripheral blood lymphocytes
Differentiating nicotine- versus schizophrenia-associated decreases of the α7 nicotinic acetylcholine receptor transcript, CHRFAM7A, in peripheral blood lymphocytes
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DOI：
10.1007/s00702-008-0164-y
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发表时间：
2009-02-01
影响因子：
3.3
通讯作者：
Yolken, Robert H.
中科院分区：
文献类型：
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作者：
Severance, Emily G.;Dickerson, Faith B.;Yolken, Robert H.
尼古丁成瘾在精神分裂症患者中很普遍。尼古丁对尼古丁受体(NAChRs)的激活是时间和剂量依赖的，但基因表达分析通常依赖于定性的自我或家庭报告的吸烟测量。我们寻找脑α7-nAChR活性的淋巴细胞替代品，并测试受体转录是否与同时测量的吸烟血清生物标记物[可替宁，C反应蛋白(CRP)]相关。检测淋巴细胞α7相关亚型的聚合酶链式反应调查发现，CHRFAM7A是唯一持续可扩增的转录本。在20名吸烟匹配的人(10名精神分裂症患者和10名对照组)中，我们发现吸烟者和吸烟者的可替宁和自我报告的CHRFAM7A显著低于不吸烟者(P
Nicotine addiction is prevalent in individuals with schizophrenia. Nicotine activation of nicotinic receptors (nAChRs) is time- and dose-dependent, but gene expression analyses often rely on qualitative self- or family-reported measures of smoking. We sought lymphocyte surrogates for cerebral alpha 7-nAChR activity and tested if receptor transcription correlated with concurrently measured serum biomarkers for smoking [cotinine, C-reactive protein (CRP)]. PCR surveys to detect lymphocytic alpha 7-related isoforms identified CHRFAM7A as the only consistently amplifiable transcript. In 20 smoking-matched people (n = 10 schizophrenia, n = 10 controls), we found significantly lower CHRFAM7A in cotinine and self-reported smokers versus nonsmokers (p