Phospholipid-linked coumarin: A fluorescent probe for sensing hydroxyl radicals in lipid membranes
Phospholipid-linked coumarin: A fluorescent probe for sensing hydroxyl radicals in lipid membranes
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DOI：
10.2116/analsci.24.293
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发表时间：
2008-02-01
影响因子：
1.6
通讯作者：
Imato, Toshihiko
中科院分区：
文献类型：
--
作者：
Soh, Nobuaki;Makihara, Koji;Imato, Toshihiko
建立了一种荧光探针DPPEC (1,2-(d) - bari (p) - bar almitoylglycero （p) - bar hosphoryi (e) - bar乙醇胺标记香豆素），用于检测脂质膜中的羟基自由基（中心点OH）。香豆素部分有助于中心点OH的荧光检测，磷脂部分则驱动探针定位在脂质膜上。脂质体膜中的DPPEC与中心点OH迅速反应，荧光强度随中心点OH浓度的增加而增加。DMSO的加入有效地抑制了中心点OH引起的荧光强度的增加。该探针对中心点OH的荧光响应高于其他活性氧，如过氧化氢、一氧化氮、过氧亚硝酸盐、烷基过氧自由基和次氯酸盐。DPPEC可作为一种定位于脂质膜的新型荧光探针，有效地检测中心点OH。
A fluorescent probe, DPPEC (1,2-(d) under bari (p) under bar almitoylglycero (p) under bar hosphoryi (e) under bar thanolamine labeled with coumarin) was developed for detecting hydroxyl radical (center dot OH) in lipid membranes. The coumarin moiety contributes to the fluorescent detection of center dot OH and the phospholipids moiety gives a driving force to localize the probe in lipid membranes. DPPEC in liposomal membranes rapidly reacted with center dot OH and increased the fluorescence intensity, depending on the concentration of center dot OH. The increase in the fluorescence intensity induced by center dot OH was effectively suppressed by the addition of DMSO. The probe exhibited a higher fluorescence response to center dot OH over other reactive oxygen species, such as hydrogen peroxide, nitric oxide, peroxynitrite, alkylperoxyl radical, and hypochlorite. DPPEC would be useful as a new type of fluorescent probe that can localize in lipid membranes and detect center dot OH efficiently.