Modern techniques for the analysis of chromatin and nuclear organization in C. elegans.
Modern techniques for the analysis of chromatin and nuclear organization in C. elegans.
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DOI：
10.1895/wormbook.1.169.1
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发表时间：
2014-04-02
期刊：
影响因子：
--
通讯作者：
Meister, Peter
中科院分区：
文献类型：
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作者：
Askjaer, Peter;Ercan, Sevinc;Meister, Peter
近年来，秀丽隐杆线虫已成为研究核结构、细胞分化和生物体发育之间关系的新模型。一方面，线虫以其固定的谱系和透明的身体是用显微镜观察体内特定细胞类型基因功能的很好的模式生物。另一方面，两种不同的技术已经被应用于线虫全基因组染色质相关蛋白的结合位点的鉴定：染色质免疫沉淀(ChIP)和Dam介导的鉴定(DamID)。我们在这里总结这三种技术，因为它们是相辅相成的。我们还强调了各个方法的优势和差异。
In recent years, Caenorhabditis elegans has emerged as a new model to investigate the relationships between nuclear architecture, cellular differentiation, and organismal development. On one hand, C. elegans with its fixed lineage and transparent body is a great model organism to observe gene functions in vivo in specific cell types using microscopy. On the other hand, two different techniques have been applied in nematodes to identify binding sites for chromatin-associated proteins genome-wide: chromatin immunoprecipitation (ChIP), and Dam-mediated identification (DamID). We summarize here all three techniques together as they are complementary. We also highlight strengths and differences of the individual approaches.