A new specialization in astrocytes: Glutamate‐ and ammonia‐induced nuclear size changes
A new specialization in astrocytes: Glutamate‐ and ammonia‐induced nuclear size changes
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DOI：
10.1002/jnr.22657
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发表时间：
2011-05
影响因子：
4.2
通讯作者：
C. Yang;Hui Li Li-Hui-Li-Li-49403914;Ye-Bo Zhou;Rui Chai;Rui Zhao;Yan Dong;Z. Xu;L. Lau;Yingge Zhang;J. Teng;Jianguo Chen;Albert Cheung Hoi Yu
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文献类型：
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作者：
C. Yang;Hui Li Li-Hui-Li-Li-49403914;Ye-Bo Zhou;Rui Chai;Rui Zhao;Yan Dong;Z. Xu;L. Lau;Yingge Zhang;J. Teng;Jianguo Chen;Albert Cheung Hoi Yu
我们观察到谷氨酸（Glu）处理的星形胶质细胞的核肿胀，这是伴随着但独立于星形胶质细胞肿胀。我们用从星形胶质细胞分离的细胞核证实了Glu诱导的细胞核肿胀。氨与Glu代谢相关，可诱导完整星形胶质细胞的核肿胀，但可诱导分离细胞核收缩。其他化合物，如谷氨酰胺，天冬氨酸，牛磺酸，甘氨酸和ATP没有引起任何核肿胀在孤立的星形胶质细胞核。令人惊讶的是，Glu和氨在培养物中的小胶质细胞、C6、HEK 293或Hep G2细胞系及其分离的细胞核中不诱导核肿胀。Glu和氨诱导的核大小变化似乎是星形胶质细胞对这两种密切相关的代谢化合物的特异性反应。© 2011 Wiley‐利斯公司
We observed nuclear swelling in glutamate (Glu)‐treated astrocytes that was concomitant with but independent of astrocytic cell swelling. We confirmed Glu‐induced nuclear swelling with nuclei isolated from astrocytes. Ammonia is metabolically related to Glu and could induce a nuclear swelling in intact astrocytes but shrinkage in isolated nuclei. Other compounds such as glutamine, aspartate, taurine, glycine, and ATP did not cause any nuclear swelling in isolated nuclei of astrocytes. Surprisingly, Glu and ammonia did not induce nuclear swelling in microglia, C6, HEK 293, or Hep G2 cell lines in cultures and their isolated nuclei. The Glu‐ and ammonia‐induced nuclear size changes appear to be a specific response of astrocytes to these two closely related metabolic compounds. © 2011 Wiley‐Liss, Inc.