Visualizing, quantifying, and manipulating mitochondrial DNA in vivo.
Visualizing, quantifying, and manipulating mitochondrial DNA in vivo.
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DOI：
10.1074/jbc.rev120.015101
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发表时间：
2020-12-18
期刊：
影响因子：
--
通讯作者：
Jones NS
中科院分区：
文献类型：
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作者：
Prole DL;Chinnery PF;Jones NS
线粒体DNA （mtDNA）编码支持线粒体功能的蛋白质和rna，从而支持许多生理过程。mtDNA的突变可引起线粒体疾病，并与衰老有关。细胞内的mtDNA在线粒体基质内被组织成类核，但细胞内mtDNA类核是如何形成和调控的还不完全清楚。细胞内mtDNA的可视化是获得机制洞察力的有力手段。操纵细胞内mtDNA的数量和序列在实验和开发治疗线粒体疾病的治疗干预措施方面是重要的。这篇综述详细介绍了实验工具和技术的最新发展和改进机会，这些工具和技术可用于可视化、量化和操纵细胞内mtDNA的特性。
Mitochondrial DNA (mtDNA) encodes proteins and RNAs that support the functions of mitochondria and thereby numerous physiological processes. Mutations of mtDNA can cause mitochondrial diseases and are implicated in aging. The mtDNA within cells is organized into nucleoids within the mitochondrial matrix, but how mtDNA nucleoids are formed and regulated within cells remains incompletely resolved. Visualization of mtDNA within cells is a powerful means by which mechanistic insight can be gained. Manipulation of the amount and sequence of mtDNA within cells is important experimentally and for developing therapeutic interventions to treat mitochondrial disease. This review details recent developments and opportunities for improvements in the experimental tools and techniques that can be used to visualize, quantify, and manipulate the properties of mtDNA within cells.