HUMAN CARDIAC TROPONIN-T - IDENTIFICATION OF FETAL ISOFORMS AND ASSIGNMENT OF THE TNNT2 LOCUS TO CHROMOSOME 1Q
HUMAN CARDIAC TROPONIN-T - IDENTIFICATION OF FETAL ISOFORMS AND ASSIGNMENT OF THE TNNT2 LOCUS TO CHROMOSOME 1Q
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DOI：
10.1006/geno.1994.1271
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发表时间：
1994-05-15
期刊：
影响因子：
4.4
通讯作者：
BARTON, PJR
中科院分区：
文献类型：
--
作者：
TOWNSEND, PJ;FARZA, H;BARTON, PJR
肌钙蛋白复合物位于横纹肌的细丝上，由三种多肽组成：肌钙蛋白T、肌钙蛋白I和肌钙蛋白C。在人类和其他脊椎动物中，已经基于分子克隆描述了三种肌钙蛋白T基因。它们以组织特异性方式表达，并编码分别在心肌、慢骨骼肌和快骨骼肌中表达的肌钙蛋白T同种型。这些基因中的每一个都受到选择性剪接，导致产生多种组织特异性同种型。我们从成人心脏中克隆了编码人心肌肌钙蛋白T的cDNA，并利用这些cDNA证明了多种心肌肌钙蛋白T mRNA存在于人胎儿心脏中，这是由于该基因5'编码区的选择性剪接所致。克隆的cDNA与基因组DNA的杂交鉴定出单拷贝基因，并且使用体细胞杂交分析，我们已经将相应的基因位点（指定为TNNT 2）定位到1号染色体的长臂（1cen-qter）。(C)1994年出版社出版。
The troponin complex is located on the thin filament of striated muscle and is composed of three component polypeptides: troponin T, troponin I, and troponin C. Three troponin T genes have been described on the basis of molecular cloning in humans and other vertebrates. These are expressed in a tissue-specific manner and encode the troponin T isoforms expressed in cardiac muscle, slow skeletal muscle, and fast skeletal muscle, respectively. Each of these genes is subject to alternative splicing, resulting in the production of multiple tissue-specific isoforms. We have cloned cDNAs encoding human cardiac troponin T from adult heart and have used these to demonstrate that multiple cardiac troponin T mRNAs are present in the human fetal heart, resulting from alternative splicing in the 5' coding region of the gene. Hybridization of the cloned cDNAs to genomic DNA identifies a single-copy gene, and using somatic cell hybrid analysis, we have mapped the corresponding gene locus (designated TNNT2) to the long arm of chromosome 1 (1cen-qter). (C) 1994 Academic Press, Inc.