Mathermycin, a Lantibiotic from the Marine Actinomycete Marinactinospora thermotolerans SCSIO 00652
Mathermycin, a Lantibiotic from the Marine Actinomycete Marinactinospora thermotolerans SCSIO 00652
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Mathermycin，一种来自海洋放线菌 Marinaactinospora thermotolerans 的羊毛硫抗生素 SCSIO 00652
DOI：
10.1128/aem.00926-17
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发表时间：
2017-08-01
影响因子：
4.4
通讯作者：
Wang, Huan
中科院分区：
文献类型：
--
作者：
Chen, Erquan;Chen, Qi;Wang, Huan
摘要羊毛硫抗生素是核糖体合成和后修饰的抗菌肽，其结构中含有硫醚键。在这项研究中，我们确定了生物合成基因簇的肉桂霉素类似物，命名为mathermycin，从海洋放线菌thermotolerans SCSIO 00652和重建其生物合成链霉菌变铅青和大肠杆菌。对马瑟霉素的关键翻译后修饰酶进行了表征。马瑟霉素表现出抗微生物活性，因此代表了来自Marinactinospora物种的肉桂霉素样羊毛硫抗生素的实例。由于细菌对当前抗生素的耐药性不断增加，迫切需要发现可用作潜在药物的新抗菌化合物。羊毛硫抗生素是重要的抗微生物化合物，已在临床环境和食品工业中得到应用。我们在这里报告的发现，一个新的羊毛硫抗生素，mathermycin，从海洋来源的Marinactinospora thermotolerans菌株和阐明其生物合成。我们还证明了马瑟霉素对芽孢杆菌菌株具有抗菌活性。
ABSTRACT Lantibiotics are antimicrobial peptides belonging to the family of ribosomally synthesized and posttranslationally modified peptides (RiPPs) and feature thioether linkages in their structures. In this study, we identified the biosynthetic gene cluster of a cinnamycin analog, named mathermycin, from Marinactinospora thermotolerans SCSIO 00652 and reconstituted its biosynthesis in Streptomyces lividans and Escherichia coli. Key posttranslational modification enzymes of mathermycin were characterized. Mathermycin exhibited antimicrobial activity and therefore represents an example of cinnamycin-like lantibiotics from Marinactinospora species. IMPORTANCE The discovery of new antimicrobial compounds that can be used as potential drugs is in urgent need due to increasing bacterial resistance to current antibiotics. Lantibiotics are important antimicrobial compounds that have found applications in both the clinic setting and food industry. We report here the discovery of a new lantibiotic, mathermycin, from a marine-derived Marinactinospora thermotolerans strain and elucidation of its biosynthesis. We also demonstrate that mathermycin possesses antimicrobial activity toward a Bacillus strain.