Genetic architecture and key genes controlling the diversity of oil composition in rice grains
Genetic architecture and key genes controlling the diversity of oil composition in rice grains
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DOI：
10.1016/j.molp.2020.12.001
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发表时间：
2021-03-01
期刊：
影响因子：
27.5
通讯作者：
He, Yuqing
中科院分区：
文献类型：
--
作者：
Zhou, Hao;Xia, Duo;He, Yuqing
稻米油是一种宝贵的营养来源。然而，水稻籽粒中油脂生物合成的遗传基础仍不清楚。在这项研究中，我们进行了一个全基因组的关联研究油成分和油浓度在一个不同的面板533栽培稻加入。11个与油分相关的性状变异较大，各亚群体间油分组成存在分化。我们确定了46个位点，显着相关的谷物油浓度或组成，其中16个被检测到在三个重组自交系群体。从这46个位点中确定了26个编码参与油代谢的酶的候选基因，其中4个（PAL 6，LIN 6，MYR 2和ARA 6）被发现有助于油成分的自然变异，并显示亚群之间的分化。有趣的是，群体遗传分析显示，在粳稻中选择了PAL 6和LIN 6的特定单倍型。基于这些结果，我们提出了一个可能的油在水稻籽粒中的生物合成途径。总的来说，我们的研究结果提供了新的见解油在水稻籽粒生物合成的遗传基础，可以促进基于标记的水稻品种的育种与提高油和粮食品质。
Rice grain oil is a valuable nutrient source. However, the genetic basis of oil biosynthesis in rice grains remains unclear. In this study, we performed a genome-wide association study on oil composition and oil concentration in a diverse panel of 533 cultivated rice accessions. High variation for 11 oil-related traits was observed, and the oil composition of rice grains showed differentiation among the subpopulations. We identified 46 loci that are significantly associated with grain oil concentration or composition, 16 of which were detected in three recombinant inbred line populations. Twenty-six candidate genes encoding enzymes involved in oil metabolism were identified from these 46 loci, four of which (PAL6, LIN6, MYR2, and ARA6) were found to contribute to natural variation in oil composition and to show differentiation among the subpopulations. Interestingly, population genetic analyses revealed that specific haplotypes of PAL6 and LIN6 have been selected in japonica rice. Based on these results, we propose a possible oil biosynthetic pathway in rice grains. Collectively, our results provide new insights into the genetic basis of oil biosynthesis in rice grains and can facilitate marker-based breeding of rice varieties with enhanced oil and grain quality.