Role of astrocytes in antigen presentation and naive T-cell activation
Role of astrocytes in antigen presentation and naive T-cell activation
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DOI：
10.1016/s0165-5728(99)00215-5
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发表时间：
2000-07-01
影响因子：
3.3
通讯作者：
Liblau, RS
中科院分区：
文献类型：
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作者：
Cornet, A;Bettelli, E;Liblau, RS
星形胶质细胞可能在中枢神经系统炎症性疾病(如MS和EAE)的抗原提呈中发挥作用。在这项研究中，我们评估了纯化的星形胶质细胞培养物是否能刺激TCR转基因小鼠的原始CD4(+)或CD8(+)T细胞。如前所述，星形胶质细胞只有在干扰素-γ存在的情况下才能维持抗原特异性的CD4(-)T细胞的增殖，干扰素-伽马能促进星形胶质细胞上MHC II和B7分子的表达。此外，我们还发现星形胶质细胞还具有向初始CD8(+)T细胞递呈抗原并促进其增殖的能力。在一个系统中，CD8(+)T细胞的增殖依赖于干扰素-γ诱导的星形胶质细胞上B7分子的上调。然而，在第二个TCR转基因系统中，星形胶质细胞即使在没有干扰素-γ的情况下也可以诱导初始CD8(+)T细胞增殖。讨论了这些发现可能对中枢神经系统炎症性疾病的病理生理学的影响。(C)2000 Elsevier Science B.V.保留所有权利。
Astrocytes may have a role in antigen presentation in inflammatory diseases of the central nervous system (CNS) such as MS and EAE. In this study, we have assessed whether purified astrocyte cultures could stimulate naive CD4(+) or CD8(+) T-cells from TCR transgenic mice. As previously described, astrocytes sustained antigen-specific CD4(-) T-cell proliferation only in the presence of IFN-gamma, which promotes expression of both MHC class II and B7 molecules on astrocytes. In addition, we show that astrocytes also have the capacity to present antigens to naive CD8(+) T-cell and promote their proliferation. In one system, this CD8(+) T-cell proliferation was dependent on IFN-gamma-induced upregulation of B7 molecules on astrocytes. However, in a second TCR transgenic system, astrocytes could induce naive CD8(+) T-cell proliferation even in the absence of IFN-gamma. The possible implications of these findings for the pathophysiology of CNS inflammatory diseases are discussed. (C) 2000 Elsevier Science B.V. All rights reserved.