Alteration of Plasma Galactose/N-acetylgalactosamine Level After Irradiation
Alteration of Plasma Galactose/N-acetylgalactosamine Level After Irradiation
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DOI：
10.1007/978-1-4614-4989-8_21
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Zhang, Lurong
中科院分区：
文献类型：
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作者：
Ma, Jun;Han, Deping;Zhang, Lurong
尽管糖蛋白在细胞内和细胞间活动中具有多种功能和结构作用，但电离辐射（IR）对糖基化的影响很大程度上未知。为了探索这种效应，我们建立了一种夹心测定法，其中 PHA-L（一种凝集白细胞的植物血凝素）用作涂层来捕获含有复杂寡糖的糖蛋白；然后测量结合的糖蛋白。 C57BL/6 小鼠暴露于 0、3、6 或 10 Gy，并在 6、12、18、24、48、72 或 168 小时收集血浆，然后分析含有半乳糖/N-乙酰半乳糖胺 (Gal/GalNAc) 的蛋白质。我们发现（1）夹心法准确测量了糖蛋白水平，（2）IR后6-12小时，含有GalNAc的糖蛋白量增加，（3）在72和168小时，10 Gy与Gal/GalNAc的减少相关。这些IR诱导的改变可能与糖蛋白释放到血液中以及与糖基化过程相关的蛋白质和基因的损伤有关。
Although glycoproteins possess a variety of functional and structural roles in intracellular and intercellular activities, the effect of ionizing radiation (IR) on glycosylation is largely unknown. To explore this effect, we established a sandwich assay in which PHA-L, a phytohaemagglutinin that agglutinates leukocytes, was used as a coating layer to capture glycoproteins containing complex oligosaccharides; the bound glycoproteins were then measured. C57BL/6 mice were exposed to 0, 3, 6, or 10 Gy, and the plasma was collected at 6, 12, 18, 24, 48, 72, or 168 h and then analyzed for galactose/N-acetylgalactosamine (Gal/GalNAc) containing proteins. We found that (1) the sandwich assay accurately measured the level of glycoproteins, (2) 6-12 h after IR, the amount of glycoproteins containing GalNAc increased, and (3) at 72 and 168 h, 10 Gy was associated with a decrease in Gal/GalNAc. These IR-induced alterations might relate to the release of glycoproteins into the blood and the damage of the proteins and genes that are related to the glycosylation process.