Identification of strains of Alcaligenes and Agrobacterium by a polyphasic approach
Identification of strains of Alcaligenes and Agrobacterium by a polyphasic approach
复制标题
DOI：
10.1128/jcm.39.9.3104-3109.2001
复制
发表时间：
2001-09-01
影响因子：
9.4
通讯作者：
Bizet, C
中科院分区：
文献类型：
--
作者：
Clermont, D;Harmant, C;Bizet, C
在土生菌属和农杆菌属中，稳定的区别性生化性状的数量有限，因此难以通过常规试验进行区分。此外，基因组研究最近大大修改了这些属的命名法；例如，Alcaligenes xylosaxidans在1998年被转移到Achromobacter属。检测了25株木糖氧化无色杆菌、3株农杆菌、5株赤潮菌和4株属于美国疾病控制和预防中心IVc-2组的未命名菌株。这些菌株通过常规测试，包括生化测试来表征。用biotype100条带研究了99种碳水化合物、有机酸和氨基酸的同化作用，用EcoRI或PstI限制性内切酶切割总DNA后，用Riboprinter微生物鉴定系统获得了rRNA基因的限制性内切模式。这种多相方法使木杉属的两个亚种得以明显分离。证实了5株菌株与拉尔斯通菌型菌株之间的关系。同样，有3株菌株与人类赭杆菌型菌株有亲缘关系。研究结果表明，底物同化试验和自动核糖分型是鉴定木氧化木单胞菌亚种的一种简单、快速、可靠的方法，这两种方法可以在缺乏鉴别生化特征的情况下作为鉴定未识别菌株的替代方法。
The number of stable discriminant biochemical characters is limited in the genera Alcaligenes and Agrobacterium, whose species are consequently difficult to distinguish from one another by conventional tests. Moreover, genomic studies have recently drastically modified the nomenclature of these genera; for example, Alcaligenes xylosaxidans was transferred to the genus Achromobacter in 1998. Twenty-five strains of Achromobacter xylosoxidans, three strains of an Agrobacterium sp., five strains of an Alcaligenes sp., and four unnamed strains belonging to the Centers for Disease Control and Prevention group IVc-2 were examined. These strains were characterized by conventional tests, including biochemical tests. The assimilation of 99 carbohydrates, organic acids, and amino acids was studied by using Biotype-100 strips, and rRNA gene restriction patterns were obtained with the automated Riboprinter microbial characterization system after cleavage of total DNA with EcoRI or PstI restriction endonuclease. This polyphasic approach allowed the two subspecies of A. xylosoxidans to be clearly separated. Relationships between five strains and the Ralstonia paucula type strain were demonstrated. Likewise, three strains were found to be related to the Ochrobactrum anthropi type strain. We showed that substrate assimilation tests and automated ribotyping provide a simple, rapid, and reliable means of identifying A. xylosoxidans subspecies and that these two methods can be used as alternative methods to characterize unidentified strains rapidly when discriminant biochemical characters are missing.