Preparation of carboxyl group-modified palladium nanoparticles in an aqueous solution and their conjugation with DNA.
Preparation of carboxyl group-modified palladium nanoparticles in an aqueous solution and their conjugation with DNA.
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DOI：
10.1039/c2nr30649b
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发表时间：
2012-05
期刊：
影响因子：
6.7
通讯作者：
Zhifei Wang;Hongying Li;Shuang Zhen;N. He
中科院分区：
文献类型：
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作者：
Zhifei Wang;Hongying Li;Shuang Zhen;N. He
近年来，纳米材料在生物分子标记及其检测中的应用引起了人们的广泛关注。由于钯纳米颗粒缺乏与DNA结合的适当表面功能，目前对钯纳米颗粒（Pd NPs）的研究很少。因此，在本文中，我们首次提出了一种方法来制备羧基修饰的钯纳米粒子（平均尺寸为6 nm），通过使用11-巯基十一烷酸（MUDA）作为稳定剂在水溶液中。研究了各种还原反应条件对Pd NPs形貌的影响。采用TEM、UV-vis、FT-IR和XPS等技术对纳米粒子进行了表征。DNA最终通过活化羧基共价结合到Pd NPs的表面，这通过琼脂糖凝胶电泳和荧光分析证实。所得Pd NPs-DNA缀合物显示出高的单碱基对错配辨别能力。本工作为钯纳米粒子在生物分析领域的进一步应用奠定了良好的基础。
The use of nanomaterials in biomolecular labeling and their corresponding detection has been attracting much attention, recently. There are currently very few studies on palladium nanoparticles (Pd NPs) due to their lack of appropriate surface functionalities for conjugation with DNA. In this paper, we thus firstly present an approach to prepare carboxyl group-modified Pd NPs (with an average size of 6 nm) by the use of 11-mercaptoundecanoic acid (MUDA) as a stabilizer in the aqueous solution. The effect of the various reducing reaction conditions on the morphology of the Pd NPs was investigated. The particles were further characterized by TEM, UV-vis, FT-IR and XPS techniques. DNA was finally covalently conjugated to the surface of the Pd NPs through the activation of the carboxyl group, which was confirmed by agarose gel electrophoresis and fluorescence analysis. The resulting Pd NPs-DNA conjugates show high single base pair mismatch discrimination capabilities. This work therefore sets a good foundation for further applications of Pd NPs in bio-analytical research.