Clostridium strain co-cultures for biohydrogen production enhancement from condensed molasses fermentation solubles
Clostridium strain co-cultures for biohydrogen production enhancement from condensed molasses fermentation solubles
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DOI：
10.1016/j.ijhydene.2009.06.028
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发表时间：
2009-09-01
影响因子：
7.2
通讯作者：
Lin, Chiu-Yue
中科院分区：
文献类型：
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作者：
Hsiao, Chin-Lang;Chang, Jui-Jen;Lin, Chiu-Yue
在水力停留时间为8 h的条件下，以40 g-COD/L的浓缩糖蜜发酵可溶物（CMS）为底物，采用厌氧连续流氢气发酵罐进行发酵。血清瓶用于种子微生物菌群培养和分批氢发酵试验（CMS底物浓度为10-160 g-COD/L）。从种子罐中分离得到3株产氢细菌Clostridium sporosphaeroides F52、Clostridium tyrobutyricum F4和Clostridium pasteurianum F40，分别作为种子菌进行单菌和混合培养，测定其产氢能力。这些菌株具有特定的氢酶基因，可以检测到从CMS喂养的氢发酵罐，是主要的氢生产者。C.巴氏杆菌F40为优势产氢菌，而C. sporosphaeroides F52可能在碳水化合物和谷氨酸的降解中起主要作用。这些菌株可以作为共生混合培养过程共培养，以提高产氢率。C. pasteurianum F40或C. tyrobutyricum F4与谷氨酸利用菌C. sporosphaeroides F52根据底物CMS浓度有效地提高了12-220%的氢气产生。(C)2009年国际氢能协会。由爱思唯尔有限公司出版。保留所有权利。
An anaerobic continuous-flow hydrogen fermentor was operated at a hydraulic retention time of 8 h using condensed molasses fermentation solubles (CMS) substrate of 40 g-COD/L. Serum bottles were used for seed micro-flora cultivation and batch hydrogen fermentation tests (CMS substrate concentrations of 10-160 g-COD/L). Three hydrogen-producing bacterial strains Clostridium sporosphaeroides F52, Clostridium tyrobutyricum F4 and Clostridium pasteurianum F40 were isolated from the seed fermentor and used as the seeding microbes in single and mixed-culture cultivations for determining their hydrogen productivity. These strains possessed specific hydrogenase genes that could be detected from CMS-fed hydrogen fermentors and were major hydrogen producers. C. pasteurianum F40 was the dominant strain with a high hydrogen production rate while C. sporosphaeroides F52 may play a main role in degrading carbohydrate and glutamate. These strains could be co-cultivated as a symbiotic mixed-culture process to enhance hydrogen productivity. C. pasteurianum F40 or C. tyrobutyricum F4 co-culture with the glutamate-utilizing strain C. sporosphaeroides F52 efficiently enhanced hydrogen production by 12-220% depending on the substrate CMS concentrations. (C) 2009 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.