IDENTIFICATION OF AN APHID SEX-PHEROMONE
IDENTIFICATION OF AN APHID SEX-PHEROMONE
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DOI：
10.1038/325614a0
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发表时间：
1987-02-12
期刊：
影响因子：
64.8
通讯作者：
WOODCOCK, CM
中科院分区：
文献类型：
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作者：
DAWSON, GW;GRIFFITHS, DC;WOODCOCK, CM
虽然蚜虫在夏季在其寄主植物上进行无性繁殖，但许多物种会迁移到冬季寄主上进行有性繁殖。某些物种的雄性通过雌性后腿释放的性引诱信息素来定位配偶1 -4。在这里，我们报告这样一个信息素的鉴定从豌豆蚜。采用气相色谱-单细胞记录技术（GC-SCR）和气相色谱-质谱联用技术（GC-MS）对雌性离体腿提取物进行了分析。通过与从天然产物中分离或合成的经鉴定的化合物进行比较，确认了通过MS分析作为单萜类化合物荆芥内酯（I）（一种已知的猫引诱剂）和荆芥内酯醇（II）的特定异构体的两种活性组分的初步鉴定。虽然在实验室生物测定中单独使用时，两种化合物均对雄性无吸引力，但两种组分的混合物产生的反应与雌性腿部提取物引起的反应相同。因此，M.蚕豆包括荆芥内酯I和荆芥内酯醇II的协同混合物。蚜虫性信息素的鉴定将为研究和控制这些普遍存在的害虫的行为提供进一步的手段。
Although aphids reproduce asexually on their host plants during the summer, many species migrate to a winter host where sexual reproduction occurs. Males of certain species locate mates by means of a sex attractant pheromone released from the hind legs of the females1–4. Here we report the identification of such a pheromone from the vetch aphidMegoura viciae. Extracts of the excised legs of sexual females were analysed by gas chromatography-coupled single cell recording techniques (GC-SCR)5and by gas chromatography-coupled mass spectrometry (GC-MS). A tentative identification by MS analysis of the two active components as specific isomers of the monoterpenoids nepetalactone (I), a known cat attractant, and nepetalactol (II) was confirmed by comparison with authenticated compounds isolated or synthesized from natural products. Although neither compound was attractive to males when presented alone in the laboratory bioassay, a mixture of the two components produced a response equal to that elicited by the female leg extract. Thus the sex pheromone ofM. viciaecomprises a synergistic mixture of the nepetalactone I and the nepetalactol II. The identification of aphid sex pheromones will provide a further means of investigating and manipulating the behaviour of these ubiquitous pests.