High-Quality CuInS2/ZnS Quantum Dots for In vitro and In vivo Bioimaging
High-Quality CuInS2/ZnS Quantum Dots for In vitro and In vivo Bioimaging
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DOI：
10.1021/cm3015594
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发表时间：
2012-08-14
影响因子：
8.6
通讯作者：
Gu, Yueqing
中科院分区：
文献类型：
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作者：
Deng, Dawei;Chen, Yuqi;Gu, Yueqing
生物相容性量子点（QD）的生物医学成像的探索是目前纳米技术发展最快的领域之一。这一策略克服了成熟的II-VI族和其他半导体U（Cd、Hg、Pb、Se、Te、As等）的固有毒性。这仍然是其临床应用的主要障碍。在本报告中，我们合成了高质量的CuInS 2/ZnS（CIS/ZnS）量子点，而不使用传统的有毒重金属。这些量子点表现出改善的光致发光（PL）性能，与可调谐的发射峰范围从550至800 nm和最大的PL量子产率（QY）高达80%。接下来，我们探索了使用可生物降解的叶酸修饰的N-琥珀酰-N '-辛基壳聚糖（FA-SOC）胶束制备的油溶性CIS/ZnS量子点的有效负载。还研究了使用体外和体内光学成像技术将所得US负载胶束靶向肿瘤的功效。结果表明，胶束平台允许这些油溶性量子点在水中的成功配方，同时保留初始CIS/ZnS量子点的形态，晶体结构和PL。这项研究表明，跨不同的空间尺度（体外细胞成像和体内小动物成像）使用生物相容性CIS/ZnS量子点的多功能性，用于生物成像应用。
The exploration of biocompatible quantum dots (QDs) for biomedical imaging is currently one of the fastest growing fields of nanotechnology. This strategy overcomes the intrinsic toxicity of well-developed II-VI and other semiconductor Us (Cd, Hg, Pb, Se, Te, As, etc.) that remains a major obstacle to their clinical use. In this report, we synthesized high-quality CuInS2/ZnS (CIS/ZnS) QDs without using conventional toxic heavy metals. These QDs exhibited improved photoluminescence (PL) properties, with tunable emission peaks ranging from 550 to 800 nm and a maximum PL quantum yield (QY) up to 80%. Next, we explored the effective loading of the prepared oil-soluble CIS/ZnS QDs using biodegradable folate-modified N-succinyl-N'-octyl chitosan (FA-SOC) micelles. Targeting efficacy of the resulting Us-loaded micelles to tumors using in vitro and in vivo optical imaging techniques was also investigated. The results show that the micelle platform allowed successful formulation of these oil-soluble QDs in water, while retaining the morphology, crystal structure, and PL of the initial CIS/ZnS QDs. This study demonstrates the versatility of using biocompatible CIS/ZnS QDs across different spatial scales (in vitro cell imaging and in vivo small animal imaging) for multicolor biological imaging applications.