Are Natural Deep Eutectic Solvents the Missing Link in Understanding Cellular Metabolism and Physiology?
Are Natural Deep Eutectic Solvents the Missing Link in Understanding Cellular Metabolism and Physiology?
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DOI：
10.1104/pp.111.178426
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发表时间：
2011-08-01
期刊：
影响因子：
7.4
通讯作者：
Verpoorte, Robert
中科院分区：
文献类型：
--
作者：
Choi, Young Hae;van Spronsen, Jaap;Verpoorte, Robert
在过去的十年里，代谢组学已经发展成为研究生物体和细胞新陈代谢的主要工具，通过这种方法，人们对代谢网络和生物体对各种外部条件的反应了解了很多(Lay等人，2006年)。这项工作的大部分涉及一些化学计量学方法，以确定各种事件的代谢组数据中的标记。但事实上，在理解代谢组数据本身的意义以及观察到的所有化合物的作用方面，几乎没有做过什么工作。研究化合物的组合本身，而不是研究观察到的化合物与任何疾病或应用的实验条件之间的相关性，有什么逻辑吗？特别是基于核磁共振的代谢组学，可以清楚地看到生物体或细胞中存在的主要化合物，并使所有主要化合物的直接定量比较成为可能。考虑到我们近年来使用我们的基于核磁共振的代谢组学(Kim等人，2010年)协议收集的所有信息，我们问自己为什么一些非常简单的分子总是在所有微生物、哺乳动物和植物细胞中大量存在。似乎这些化合物必须在活细胞和生物体中发挥一些基本功能。这些化合物包括糖、一些氨基酸、胆碱和一些有机酸，如苹果酸、柠檬酸、乳酸和琥珀酸。除了糖，它可以作为储存产品和能量来源，其他化合物的存在数量如此之大，以至于没有理由只将它们视为代谢途径中的中间体。
Over the past decade, metabolomics has developed into a major tool for studying the metabolism of organisms and cells, and through this approach much has been learned about metabolic networks and the reactions of organisms to various external conditions (Lay et al., 2006). Most of this work involves a number of chemometric methods to identify markers in the metabolomic data for various events. But in fact, little work has been done on understanding the meaning of the metabolomic data itself and the role of the total of the compounds observed. Is there any logic in the studying the combination of compounds itself, instead of looking at the correlations between the compounds observed and any disease or applied experimental conditions? NMR-based metabolomics in particular give a clear view of the major compounds present in an organism or cell and enable the direct quantitative comparison of all major compounds. Considering all the information we have collected in recent years using our protocol for NMR-based metabolomics (Kim et al., 2010), we asked ourselves why a few very simple molecules are always present in considerable amounts in all microbial, mammalian, and plant cells. It seems that these compounds must serve some basic function in living cells and organisms. These compounds include sugars, some amino acids, choline, and some organic acids such as malic acid, citric acid, lactic acid, and succinic acid. With the exception of sugars, which may serve as storage products and a source of energy, the other compounds are present in such large amounts that it does not make sense to consider them as only intermediates in metabolic pathways.