Late cornified envelope family in differentiating epithelia - Response to calcium and ultraviolet irradiation
Late cornified envelope family in differentiating epithelia - Response to calcium and ultraviolet irradiation
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DOI：
10.1111/j.0022-202x.2005.23699.x
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发表时间：
2005-05-01
影响因子：
6.5
通讯作者：
Byrne, C
中科院分区：
文献类型：
--
作者：
Jackson, B;Tilli, CMLJ;Byrne, C
人类一号染色体(小鼠三号染色体)上表皮分化复合体中的晚期角化包膜(LCE)基因簇包含多个编码角质层蛋白的保守基因。在LCE簇中，基因根据染色体位置和蛋白质同源性形成“组”。我们将最近被接受的LCE簇(以前的XP5，富含脯氨酸的小分子包膜蛋白基因)的命名与基因结构、分组和染色体组织联系起来，并在各种组织和环境条件下进行泛簇定量表达分析。这一分析表明：(I)该簇组织成两个“皮肤”表达组和第三个组，在所有被测试的屏障形成上皮(包括内上皮)中具有低水平的组织特异性表达模式；(Ii)LCE基因对环境刺激，如钙水平和紫外线(UV)光做出“组式”反应，突出组的功能意义；(Iii)响应UV刺激，单个正常静止的非皮肤LCE基因大量上调；以及(Iv)缺乏LCE皮肤基因表达的个体之间存在异质性，没有明显的皮肤病，表明LCE基因影响皮肤功能的微妙属性。这一定量和泛聚类表达分析表明，LCE组具有不同的功能，在组内，监管多样化允许对环境挑战做出特定的反应。
The late cornified envelope (LCE) gene cluster within the epidermal differentiation complex on human chromosome one (mouse chromosome three) contains multiple conserved genes encoding stratum-corneum proteins. Within the LCE cluster, genes form "groups" based on chromosomal position and protein homology. We link a recently accepted nomenclature for the LCE cluster (formerly XP5, small proline-rich-like, late-envelope protein genes) to gene structure, groupings, and chromosomal organization, and carry out a pan-cluster quantitative expression analysis in a variety of tissues and environmental conditions. This analysis shows that (i) the cluster organizes into two "skin" expressing groups and a third group with low-level, tissue-specific expression patterns in all barrier-forming epithelia tested, including internal epithelia; (ii) LCE genes respond "group-wise" to environmental stimuli such as calcium levels and ultraviolet (UV) light, highlighting the functional significance of groups; (iii) in response to UV stimulation there is massive upregulation of a single, normally quiescent, non-skin LCE gene; and (iv) heterogeneity occurs between individuals with one individual lacking expression of an LCE skin gene without overt skin disease, suggesting LCE genes affect subtle attributes of skin function. This quantitative and pan-cluster expression analysis suggests that LCE groups have distinct functions and that within groups regulatory diversification permits specific responsiveness to environmental challenge.