Nanotextured titanium surfaces for enhancing skin growth on transcutaneous osseointegrated devices
Nanotextured titanium surfaces for enhancing skin growth on transcutaneous osseointegrated devices
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DOI：
10.1016/j.actbio.2009.12.016
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发表时间：
2010-06-01
影响因子：
9.7
通讯作者：
Webster, Thomas J.
中科院分区：
文献类型：
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作者：
Puckett, Sabrina D.;Lee, Phin Peng;Webster, Thomas J.
经皮骨整合植入物的一个主要问题是感染，主要是由于植入物-皮肤界面的不当闭合。因此，需要研究更好地促进这些出口部位周围皮肤生长的经皮骨整合器械的设计，如果成功，将明显限制感染。由于骨科植入物已经取得了成功，因此开发具有生物启发纳米特征的表面是经皮器械需要研究的设计标准。因此，本研究检查了通过电子束蒸发和阳极氧化产生的纳米纹理钛（Ti）对角质形成细胞（皮肤形成细胞）功能的影响。电子束蒸发产生钛表面纳米功能，而阳极氧化产生钛表面纳米管。传统的钛表面主要是微米级粗糙，几乎没有纳米级的表面特征。结果显示，增加角质形成细胞粘附，除了增加角质形成细胞扩散和差异，角质形成细胞丝状伪足延伸的纳米纹理钛表面制备的电子束蒸发或阳极氧化相比，其传统的，未改性的对应物4小时后。结果进一步显示，与阳极化纳米管和未改性Ti表面相比，仅在通过电子束蒸发制备的纳米粗糙Ti表面上的角质形成细胞增殖和细胞扩散在3天和5天内增加。因此，这项体外研究的结果提供了第一个证据，即纳米改性技术应进一步研究，作为一种可能改善皮肤生长的手段，从而改善经皮骨整合骨科植入物的寿命。(C)2009 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
A major problem with transcutaneous osseointegrated implants is infection, mainly due to improper closure of the implant-skin interface. Therefore, the design of transcutaneous osseointegrated devices that better promote skin growth around these exit sites needs to be examined and, if successful, would clearly limit infection. Due to the success already demonstrated for orthopedic implants, developing surfaces with biologically inspired nanometer features is a design criterion that needs to be investigated for transcutaneous devices. This study therefore examined the influence of nanotextured titanium (Ti) created through electron beam evaporation and anodization on keratinocyte (skin-forming cell) function. Electron beam evaporation created Ti surfaces with nanometer features while anodization created Ti surfaces with nanotubes. Conventional Ti surfaces were largely micron rough, with few nanometer surface features. Results revealed increased keratinocyte adhesion in addition to increased keratinocyte spreading and differences in keratinocyte filopodia extension on the nanotextured Ti surfaces prepared by either electron beam evaporation or anodization compared to their conventional, unmodified counterparts after 4 h. Results further revealed increased keratinocyte proliferation and cell spreading over 3 and 5 days only on the nanorough Ti surfaces prepared by electron beam evaporation compared to both the anodized nanotubular and unmodified Ti surfaces. Therefore, the results from this in vitro study provided the first evidence that nano-modification techniques should be further researched as a means to possibly improve skin growth, thereby improving transcutaneous osseointegrated orthopedic implant longevity. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.