INTERLEUKIN-4 MEDIATES AUTOCRINE GROWTH OF HELPER T-CELLS AFTER ANTIGENIC-STIMULATION
INTERLEUKIN-4 MEDIATES AUTOCRINE GROWTH OF HELPER T-CELLS AFTER ANTIGENIC-STIMULATION
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DOI：
10.1073/pnas.83.24.9689
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发表时间：
1986-12-01
影响因子：
11.1
通讯作者：
VITETTA, ES
中科院分区：
文献类型：
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作者：
FERNANDEZBOTRAN, R;SANDERS, VM;VITETTA, ES
研究了白细胞介素4（IL-4）（以前称为B细胞刺激因子1）在T辅助细胞（TH）对抗原特异性B细胞或脾粘附细胞呈递的抗原的应答中的作用。无论是钥孔血蓝蛋白特异性TH细胞系或两个钥孔血蓝蛋白特异性T细胞克隆的抗原刺激导致IL-4的分泌，但不是白细胞介素2（IL-2）。在抗原刺激后6-8小时，在培养上清液中首次检测到IL-4的分泌。IL-4分泌的诱导是抗原特异性的和主要组织相容性复合体限制性的。抗原刺激也导致TH细胞对外源性添加或内源性产生的IL-4的反应性增加。抗原诱导的TH细胞增殖可被抗IL-4抗体抑制，但不被抗IL-2受体抗体抑制。这些结果表明，IL-4介导的抗原诱导的自分泌机制的一些TH细胞的增殖。结合过去的结果，这些数据表明，在涉及某些可溶性蛋白抗原的T细胞-B细胞相互作用中，IL-4而不是IL-2是激活静息B细胞和诱导TH细胞增殖的关键淋巴因子。
The role of interleukin 4 (IL-4) (previously called B-cell stimulatory factor 1) in the response of T helper (TH) cells to antigen presented by antigen-specific B cells or splenic adherent cells was investigated. Antigenic stimulation of either a keyhole-limpet-hemocyanin-specific TH-cell line or two keyhole-limpet-hemocyanin-specific T-cell clones resulted in the secretion of IL-4 but not interleukin 2 (IL-2). The secretion of IL-4 was first detected in the culture supernatant 6-8 hr after antigenic stimulation. Induction of IL-4 secretion was antigen specific and major histocompatibility complex restricted. Antigen stimulation also resulted in increased responsiveness of the TH cells to exogenously added or endogenously produced IL-4. The antigen-induced proliferation of the TH cells could be inhibited by an anti-IL-4 antibody but not by an anti-IL-2-receptor antibody. These results suggest that IL-4 mediates the proliferation of some TH cells by an antigen-induced autocrine mechanism. Taken together with past results, these data indicate that, during T-cell-B-cell interactions involving some soluble protein antigens, IL-4 and not IL-2 is the critical lymphokine for activating resting B cells and inducing proliferation of the TH cells.