Transcriptome-wide distribution and function of RNA hydroxymethylcytosine
Transcriptome-wide distribution and function of RNA hydroxymethylcytosine
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DOI：
10.1126/science.aac5253
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发表时间：
2016-01-15
期刊：
影响因子：
56.9
通讯作者：
Fuks, Francois
中科院分区：
文献类型：
--
作者：
Delatte, Benjamin;Wang, Fei;Fuks, Francois
羟甲基胞嘧啶在 DNA 中得到了很好的描述，也存在于 RNA 中。在这里，我们发现羟甲基胞嘧啶优先标记聚腺苷酸化 RNA，并由 Tet 沉积在果蝇中。我们绘制了转录组范围内的羟甲基化图谱，揭示了许多基因转录本中的羟甲基胞嘧啶，特别是编码序列中的羟甲基胞嘧啶，并确定了羟甲基化的共有位点。我们发现 RNA 羟甲基化有利于 mRNA 翻译。研究发现果蝇大脑中 Tet 和羟甲基化 RNA 最为丰富，缺乏 Tet 的果蝇大脑发育受损，同时 RNA 羟甲基化水平降低。这项研究强调了胞嘧啶羟甲基化的分布、定位和功能，并确定了果蝇中这种修饰的核心作用。
Hydroxymethylcytosine, well described in DNA, occurs also in RNA. Here, we show that hydroxymethylcytosine preferentially marks polyadenylated RNAs and is deposited by Tet in Drosophila. We map the transcriptome-wide hydroxymethylation landscape, revealing hydroxymethylcytosine in the transcripts of many genes, notably in coding sequences, and identify consensus sites for hydroxymethylation. We found that RNA hydroxymethylation can favor mRNA translation. Tet and hydroxymethylated RNA are found to be most abundant in the Drosophila brain, and Tet-deficient fruitflies suffer impaired brain development, accompanied by decreased RNA hydroxymethylation. This study highlights the distribution, localization, and function of cytosine hydroxymethylation and identifies central roles for this modification in Drosophila.