Schistosoma mansoni: production of cercarial eicosanoids as correlates of penetration and transformation.
Schistosoma mansoni: production of cercarial eicosanoids as correlates of penetration and transformation.
复制标题
曼氏血吸虫：尾蚴类二十烷酸的产生与渗透和转化相关。
作者：
A. Fusco;B. Salafsky;K. Delbrook
开发了一种方法，使用 0.25% 琼脂基质，掺入不同浓度的亚油酸盐，并在体外关联尾蚴转化和类二十烷酸的产生。刺激曼氏血吸虫尾蚴在较宽的亚油酸盐浓度范围内渗透；然而，转变过程发生在一个狭窄的范围内。在对照（无亚油酸盐）和 0.003 mM 亚油酸盐中，大约 25% 的尾蚴穿透琼脂基质。渗透率逐渐上升，直到亚油酸盐浓度为 0.3 mM 或更高时，渗透率接近 100%。直到琼脂中的亚油酸浓度达到 2.0 mM (3.8%) 时，转化过程才开始，并在 3.0 mM 时达到最大值 (91%)。浓度为 9.0 mM 的亚油酸盐具有 100% 的渗透率和转化率，但渗透很浅，尾蚴无法存活。尾蚴类二十烷酸的产生与浓度相关。各种类二十烷酸与尾蚴穿透和转化有关。渗透率与白三烯（LT，R = 0.9541）和羟基二十碳四烯酸（HETE，R = 0.8363）水平的增加相关，而转化率与前列腺素水平的增加相关（R = 0.9225）。将类二十烷酸的产生与渗透和转化率相关联，强化了这样的假设：成功的尾蚴渗透和转化取决于皮肤必需脂肪酸水平和由此产生的尾蚴类二十烷酸的产生。
A method was developed, using a 0.25% agar matrix, to incorporate varying concentrations of linoleate and correlate cercarial transformation and eicosanoid production in vitro. Schistosoma mansoni cercariae were stimulated to penetrate over a wide range of linoleate concentrations; however, the transformation process occurred over a narrow range. Approximately 25% of cercariae penetrated the agar matrix in controls (no linoleate) and 0.003 mM linoleate. Penetration rates rose gradually until, at linoleate concentrations of 0.3 mM or greater, penetration approached 100%. The transformation process did not begin until the linoleate concentration in agar reached 2.0 mM (3.8%), and achieved maximum (91%) at 3.0 mM. A concentration of 9.0 mM linoleate gave 100% penetration and transformation rates, but penetration was superficial and cercariae were not viable. Cercarial eicosanoid production was concentration-related. Various eicosanoid classes were associated with cercarial penetration and transformation. Penetration rates were correlated with increasing leukotriene (LT, R = 0.9541) and hydroxyeicosatetraenoic acid (HETE, R = 0.8363) levels, while transformation rates correlated with increasing prostaglandin levels (R = 0.9225). Correlating eicosanoid production with penetration and transformation rates strengthened the hypothesis that successful cercarial penetration and transformation are dependent on both skin essential fatty acid levels and resulting cercarial eicosanoid production.