Development of nuclear SSRs for the insular shrub Clerodendrum izuinsulare (Verbenaceae) and the widespread C. trichotomum.
Development of nuclear SSRs for the insular shrub Clerodendrum izuinsulare (Verbenaceae) and the widespread C. trichotomum.
复制标题
为岛灌木 Clerodendrum izuinsulare（马鞭草科）和广泛分布的 C. trichotomum 开发核 SSR。
DOI：
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
Y. Isagi
中科院分区：
文献类型：
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作者：
Leiko Mizusawa;S. Kaneko;M. Hasegawa;Y. Isagi
PREMISE OF THE STUDY
Nuclear microsatellite primers were developed in the insular shrub Clerodendrum izuinsulare and the closely related widespread C. trichotomum to provide molecular tools to undertake a comparative study of the reproductive systems and genetic structures of the two Clerodendrum species.
METHODS AND RESULTS
Using an improved protocol for isolating codominant compound microsatellite markers, 19 primer sets were developed in C. izuinsulare and C. trichotomum. The primers amplified dinucleotide repeats with one to nine alleles per locus in C. izuinsulare and one to 15 alleles per locus in C. trichotomum. Tests of cross-amplification showed that four to 16 loci could be amplified using these markers in five other species of Clerodendrum that were studied.
CONCLUSIONS
The microsatellite markers described here will be useful for comparative study of the reproductive systems and genetic structures of the narrow endemic C. izuinsulare and the widespread C. trichotomum.
