Establishment of consomic strains derived from A/J and SM/J mice for genetic analysis of complex traits
Establishment of consomic strains derived from A/J and SM/J mice for genetic analysis of complex traits
复制标题
DOI：
10.1007/s00335-012-9435-x
复制
发表时间：
2012-12-01
期刊：
影响因子：
2.5
通讯作者：
Nishimura, Masahiko
中科院分区：
文献类型：
--
作者：
Ohno, Tamio;Hata, Keiko;Nishimura, Masahiko
同染色体菌株，其中一条染色体来自供体菌株，其他染色体来自受体菌株，为解剖复杂的遗传性状提供了有力的工具。本研究以SM/J菌株为供体，A/J菌株为受体，建立了A-2（SM）、A-6（SM）、A-11（SM）、A-12（SM）、A-13（SM）、A-15（SM）、A-17（SM）、A-18（SM）、A-19（SM）、A- y -SM 10株经济菌株；这些是我们之前开发的一组SMXA重组自交系（RI）菌株的亲本菌株。我们分析了经济菌株和亲本菌株的体重和血脂水平。各性状的平均值在经济品系中表现出连续的变化范围，表明它们受多个基因控制。我们之前在SMXA RI株和（SM/J x A/J）F-2小鼠的6号染色体上发现了与体重有关的qtl。a -6（SM）经济品系的平均体重显著低于a /J品系，这支持了该QTL在6号染色体上的存在。同样，a -11（SM）菌株较高的血甘油三酯水平表明在11号染色体上存在先前定位的QTL， a -12（SM）菌株提供了在12号染色体上存在血总胆固醇水平QTL的证据。这些经济菌株，连同先前从A/J和SM/J小鼠中开发的SMXA RI菌株，为分析小鼠的复杂遗传性状提供了宝贵和强大的资源。
Consomic strains, in which one chromosome is derived from a donor strain and the other chromosomes are derived from the recipient strain, provide a powerful tool for the dissection of complex genetic traits. In this study we established ten consomic strains (A-2(SM), A-6(SM), A-11(SM), A-12(SM), A-13(SM), A-15(SM), A-17(SM), A-18(SM), A-19(SM), A-Y-SM) using the SM/J strain as the donor and the A/J strain as the recipient; these are the parental strains of a set of SMXA recombinant inbred (RI) strains that we had developed previously. We analyzed body weights and blood lipid levels in the consomic and parental strains. The mean values for each trait showed a continuous range of variation in the consomic strains suggesting that they are controlled by multiple genes. We previously identified suggestive QTLs for body weight on chromosome 6 in SMXA RI strains and (SM/J x A/J)F-2 mice. The observation that the A-6(SM) consomic strain had a significantly lower mean body weight than the A/J strain supports the presence of this QTL on chromosome 6. Similarly, the higher blood triglyceride level in the A-11(SM) strain shows the existence of a previously mapped QTL on chromosome 11, and the A-12(SM) strain provides evidence of a QTL for blood total cholesterol level on chromosome 12. These consomic strains, along with the previously developed set of SMXA RI strains from A/J and SM/J mice, offer an invaluable and powerful resource for the analysis of complex genetic traits in mice.