LACK OF EVIDENCE FOR INSITU FLUORESCENT PIGMENT PRODUCTION BY PSEUDOMONAS-SYRINGAE PV SYRINGAE ON BEAN LEAF SURFACES
LACK OF EVIDENCE FOR INSITU FLUORESCENT PIGMENT PRODUCTION BY PSEUDOMONAS-SYRINGAE PV SYRINGAE ON BEAN LEAF SURFACES
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DOI：
10.1094/phyto-77-1449
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发表时间：
1987-10-01
期刊：
影响因子：
3.2
通讯作者：
LINDOW, SE
中科院分区：
文献类型：
--
作者：
LOPER, JE;LINDOW, SE
荧光铁载体的假单胞菌pv。P.s. PV.黄曲霉属）是有效保护生产细胞免于暴露于紫外光（UV）的发色团（. - 254）在文化中。P. S. PV.引起菜豆[菜豆]褐斑病的菜豆属菌株B728 a产生荧光色素，并在补充有乙二胺二（邻羟基苯乙酸）（EDDA）的缺铁King's B培养基（KBM）上生长。菌株I-1是甲磺酸乙酯诱变后获得的B728 a的非荧光（Flu-）衍生物，在KBM-EDDA培养基上不生长。菌株I-1比出发菌株B728 a对紫外线辐射更敏感。根据紫外线杀灭曲线计算的LD 50值为142.4 ± 0.05。1.4和100.8 ±。1.8 erg/mm ~ 2。生长速度，固定附生种群的大小，褐斑病变的数量，和紫外线的敏感性B728 a和I-1没有显着不同的温室生长的豆类植物的叶表面。在田间生长的菜豆植物上，在7天的时间内，在四种亲本和四种流感衍生菌株之间没有观察到它们在菜豆叶表面上的种群大小的一致差异。这些间接研究没有提供P. s.原位产生荧光铁载体的证据。PV.或其对P. s.的生长、存活期或致病性的贡献。PV.豆叶表面的叶藓。
The fluorescent siderophore of Pseudomonas syringae pv. syringae (P.s. pv. syringae) is a chromophore effective in protecting the producing cells from exposure to ultraviolet light (UV) (.lambda. - 254) in culture. P. s. pv. syringae strain B728a, which causes brown spot disease of bean [Phaseolus vulgaris] produces a fluorescent pigment and grows on the iron-deficient King''s B medium (KBM) supplemented with ethylenediaminedi(o-hydroxyphenylacetic acid) (EDDA). Strain I-1, a nonfluorescent (Flu-) derivative of B728a obtained after ethyl methanesulfonate mutagenesis, did not grow on the KBM-EDDA medium. Strain I-1 was more sensitive than the parental strain B728a to UV irradiation in culture. LD50 values, calculate for UV killing curves, were 142.4 .+-. 1.4 and 100.8 .+-. 1.8 erg/mm2 for strains B728a and I-1, respectively. Growth rates, stationary epiphytic population sizes, number of brown spot lesions, and UV sensitivities of B728a and I-1 did not differ significantly on leaf surfaces of greenhouse-grown bean plants. No consistent differences were observed between four parental and four Flu- derivative strains with respect to their populations sizes on bean leaf surfaces over a 7-day period on field-grown bean plants. These indirect studies provide no evidence of in situ fluorescent siderophore production by P. s. pv. syringae or its contribution to the growth, durvival, or pathogenicity of P. s. pv. syringae on bean leaf surfaces.