Zinc treatment ameliorates diarrhea and intestinal inflammation in undernourished rats.
Zinc treatment ameliorates diarrhea and intestinal inflammation in undernourished rats.
复制标题
锌治疗可改善营养不良大鼠的腹泻和肠道炎症。
DOI：
10.1186/1471-230x-14-136
复制
发表时间：
2014-08-05
影响因子：
2.4
通讯作者：
Oriá RB
中科院分区：
文献类型：
--
作者：
de Queiroz CA;Fonseca SG;Frota PB;Figueiredo IL;Aragão KS;Magalhães CE;de Carvalho CB;Lima AÂ;Ribeiro RA;Guerrant RL;Moore SR;Oriá RB
世卫组织指南建议补充锌作为发展中国家儿童腹泻的关键辅助治疗，但锌的抗腹泻作用仍然只有部分了解。最近，人们已经认识到，低度炎症可能会影响发育迟缓。在这项研究中，我们研究了口服锌补充剂是否可以改善营养不良的断奶大鼠的体重，肠道炎症和腹泻。使用巴西东北部地区饮食（RBD）使大鼠营养不良两周，随后在最后7天用饱和乳糖溶液（30 g/kg）经口灌胃以诱导渗透性腹泻。摄入乳糖后，每天检查动物是否腹泻。抽取血液以通过原子吸收光谱法测量血清锌水平。对大鼠实施安乐死以收获空肠组织用于组织学和通过ELISA的细胞因子谱。在一个动物亚组中，在无菌条件下收获脾脏样品以定量细菌移位。口服锌补充剂增加乳糖诱导的渗透性腹泻后血清锌水平。在营养不良的大鼠中，锌改善了渗透性腹泻后的体重增加，并在摄入乳糖的第三天显著降低了空肠评分（p < 0.05），改善了空肠组织学（p < 0.0001）。与未经处理的激发对照组相比，锌补充仅在乳糖激发的营养不良大鼠中减少细菌移位（p = 0.03），并将肠道IL-1β和TNF-α细胞因子降低至对照水平。总之，我们的研究结果提供了新的机制，锌的行动，在设置腹泻和营养不良，并支持使用锌，以防止营养不良和腹泻的恶性循环。
WHO guidelines recommend zinc supplementation as a key adjunct therapy for childhood diarrhea in developing countries, however zinc’s anti-diarrheal effects remain only partially understood. Recently, it has been recognized that low-grade inflammation may influence stunting. In this study, we examined whether oral zinc supplementation could improve weight, intestinal inflammation, and diarrhea in undernourished weanling rats. Rats were undernourished using a northeastern Brazil regional diet (RBD) for two weeks, followed by oral gavage with a saturated lactose solution (30 g/kg) in the last 7 days to induce osmotic diarrhea. Animals were checked for diarrhea daily after lactose intake. Blood was drawn in order to measure serum zinc levels by atomic absorption spectroscopy. Rats were euthanized to harvest jejunal tissue for histology and cytokine profiles by ELISA. In a subset of animals, spleen samples were harvested under aseptic conditions to quantify bacterial translocation. Oral zinc supplementation increased serum zinc levels following lactose-induced osmotic diarrhea. In undernourished rats, zinc improved weight gain following osmotic diarrhea and significantly reduced diarrheal scores by the third day of lactose intake (p < 0.05), with improved jejunum histology (p < 0.0001). Zinc supplementation diminished bacterial translocation only in lactose-challenged undernourished rats (p = 0.03) compared with the untreated challenged controls and reduced intestinal IL-1β and TNF-α cytokines to control levels. Altogether our findings provide novel mechanisms of zinc action in the setting of diarrhea and undernutrition and support the use of zinc to prevent the vicious cycle of malnutrition and diarrhea.