Induction of hyperglycaemia in zebrafish (Danio rerio) leads to morphological changes in the retina
Induction of hyperglycaemia in zebrafish (Danio rerio) leads to morphological changes in the retina
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DOI：
10.1007/s00592-007-0257-3
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发表时间：
2007-09-01
影响因子：
3.8
通讯作者：
Arneson, L. S.
中科院分区：
文献类型：
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作者：
Gleeson, M.;Connaughton, V.;Arneson, L. S.
糖尿病每年影响超过1600万美国人，导致高血糖症和微血管并发症，包括视网膜病变、神经病变和肾病。已经开发了动物模型来研究1型糖尿病的免疫学方面和与糖尿病视网膜病变相关的致病机制，但是糖尿病诱导方法引起了对这些模型的关注。斑马鱼（Danio rerio）已被广泛用于研究发育过程，并且突变的斑马鱼品系已被用于检查存在于人类中的视觉疾病。在本文中，我们已经诱导高血糖症的斑马鱼，交替浸泡在葡萄糖溶液或水。将来自未处理的鱼或暴露于交替的葡萄糖/水溶液28天的鱼的眼睛解剖、切片并染色以使视网膜中的细胞体可视化。在未处理的鱼视网膜中，内丛状层（IPL）和内核层（INL）的厚度大致相同，而在反复暴露于葡萄糖溶液的鱼中，IPL的厚度约为INL的55%。与未处理的鱼相比，处理的鱼的视网膜中的IPL和INL均显著降低，这与在其他糖尿病动物模型和糖尿病患者中观察到的相似。这些结果表明，斑马鱼可用作研究糖尿病视网膜病变的动物模型。
Diabetes affects over 16 million Americans yearly, resulting in hyperglycaemia and microvascular complications, including retinopathy, neuropathy and nephropathy. Animal models have been developed to examine the immunological aspects of type 1 diabetes and the pathogenic mechanisms associated with diabetic retinopathy, but the methods of diabetes induction raise concerns regarding these models. Zebrafish (Danio rerio) have been used extensively to study developmental processes and mutant zebrafish strains have been used to examine vision disease present in humans. In this paper, we have induced hyperglycaemia in zebrafish by alternately immersing the fish in glucose solution or water. Eyes from untreated fish or fish exposed to alternating glucose/water solutions for 28 days were dissected, sectioned and stained to visualise cell bodies in the retina. In untreated fish retinas, the inner plexiform layer (IPL) and inner nuclear layer (INL) were approximately the same thickness, whereas in fish repeatedly exposed to glucose solutions the IPL was approximately 55% the thickness of the INL. Both the IPL and INL were significantly reduced in retinas of treated fish, compared to untreated fish, similar to that seen in other animal models of diabetes and in diabetic patients. These results suggest that zebrafish may be used as an animal model in which to study diabetic retinopathy.