Antioxidative Protective Effect of Icariin on the FeSO4/H2O2-damaged Human Sperm Based on Confocal Raman Micro-spectroscopy
Antioxidative Protective Effect of Icariin on the FeSO4/H2O2-damaged Human Sperm Based on Confocal Raman Micro-spectroscopy
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基于共焦拉曼显微光谱研究淫羊藿苷对FeSO4/H2O2损伤的人类精子的抗氧化保护作用
DOI：
10.1007/s11596-014-1348-3
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发表时间：
2014-10-01
影响因子：
--
通讯作者：
Chen, Jun
中科院分区：
文献类型：
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作者：
Huang, Zhan-sen;Xiao, Heng-jun;Chen, Jun
氧化应激与男性不育有关，在25%的不育男性中检测到显著较高的活性氧。虽然包括中药在内的各种替代药物的不同药物已经尝试取得了不同的成功，但关于草药或补充剂是否以及多少可能有助于提高精子的抗氧化能力的证据仍然有限。本文研究了淫羊藿甙对人精子的抗氧化作用。制备FeSO_4/H_2 O_2损伤的人精子，与淫羊藿苷体外共培养，利用显微拉曼光谱观察精子的变化。结果表明，使用514 nm激发激光的拉曼映射可以清楚地区分人精子细胞的核、颈，特别是富含精子的中间部分。通过在毫秒内获得的局域拉曼光谱特征定量分析了淫羊藿苷对精子不同细胞器的影响，结果表明淫羊藿苷能够保持人精子的“拉曼指纹”与对照组相同，表明淫羊藿苷能够保护人精子免受FeSO 4/H2 O2的损伤。淫羊藿苷可作为一种草药来源的滋补和补充剂，用于增强生殖功能。
Oxidative stress is implicated in male infertility and significantly higher reactive oxygen species are detected in 25% of infertile males. Although different agents of various alternative medicines, including traditional Chinese medicine, have been tried with varying success, evidence remains limited on whether and how much herbs or supplements might help increase the anti-oxidant ability of the sperm. This study examined the anti-oxidative effects of icariin, a flavonoid isolated fromHerbaEpimedii, on the human sperm. We prepared the FeSO4/H2O2-damaged human sperms, which were co-cultured with icariinin vitro, and then observed the changes of the sperm by employing Raman micro-spectroscopy. The results showed that Raman mapping with a 514 nm excitation laser allowed clear differentiation of the nucleus, neck, and, in particular, the mitochondria-rich middle piece of a human sperm cell. The effect of icariin on different organelles of the sperm was quantified by localized spectral Raman signatures obtained within milli-seconds, and icariin could keep the “Raman fingerprint” of the human sperm the same as the control groups, suggesting that icariin could protect the human sperm from being damaged by FeSO4/H2O2. Icariin may serve as a tonifying and replenishing agent of herbal origin for enhancing reproductive functions.