DNA amplification and expression of FADD in oral squamous cell carcinoma
DNA amplification and expression of FADD in oral squamous cell carcinoma
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DOI：
10.1111/j.1600-0714.2009.00847.x
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发表时间：
2010-08-01
影响因子：
3.3
通讯作者：
Omura, Ken
中科院分区：
文献类型：
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作者：
Prapinjumrune, Chanwit;Morita, Kei-ichi;Omura, Ken
背景：Fas相关的死亡结构域蛋白，FADD，是一种传递凋亡信号的接头蛋白。然而，最近的研究表明，FADD在细胞周期的生长和调节中也起着重要作用。为探讨FADD在口腔鳞状细胞癌中的作用，采用实时荧光定量PCR（real-time PCR）检测30例舌鳞癌组织中FADD的DNA扩增，免疫组化法检测60例舌鳞癌组织中FADD的蛋白表达。FADD表达水平与匹配的相邻上皮相比显著增加（P = 0.000）。FADD的阳性表达与SCC的淋巴结转移（P = 0.029）和5年生存率（P = 0.049）显著相关。FADD表达水平与组织病理学分化程度之间的正相关被发现仅限于T1 SCC（P = 0.019）。30例舌鳞状细胞癌中FADD的表达与DNA扩增呈中度相关（r = 0.406，P = 0.026）。结论：舌鳞状细胞癌中FADD的表达高于癌旁组织，可能与11q13.3基因扩增有关。因此，表达FADD的SCC细胞可能更容易转移并降低生存率。
Background:The Fas-associated death domain-containing protein, FADD, is an adaptor for relaying apoptotic signals. However, recent studies have shown that FADD also plays an important role in the growth and regulation of the cell cycle. The purpose of this study was to elucidate the role of FADD in oral squamous cell carcinoma (SCC).Methods:The DNA amplification of FADD from 30 samples of tongue SCC was analyzed using real-time PCR and the protein expression of FADD from 60 samples of tongue SCC was analyzed using immunohistochemistry.Results:The DNA amplifications of FADD were observed in 13 cases (44.3%) and were significantly correlated with the histopathological differentiation grade of SCCs (P = 0.009). FADD expression levels compared with the matched adjacent epithelium increased significantly (P = 0.000). Additionally, the positive expressions of FADD were significantly correlated with lymph node metastasis of SCCs (P = 0.029) and the 5-year disease-specific survival rates (P = 0.049). A positive association between FADD expression level and the histopathological differentiation grade was found to be limited to T1 SCCs (P = 0.019). DNA amplification was moderately correlated (correlation coefficient = 0.406, P = 0.026) with expression of FADD in 30 samples of tongue SCC.Conclusion:In tongue SCCs, the expression of FADD was higher when compared with that of adjacent areas, which might be determined via genomic amplification in 11q13.3. Thus, SCC cells with the expression of FADD are possibly more likely to become metastatic and to worsen survival rates.