Polymorphism of apyrimidinic DNA structures in the nucleosome.
Polymorphism of apyrimidinic DNA structures in the nucleosome.
复制标题
核小体中阿吡啶DNA结构的多态性。
DOI：
10.1038/srep41783
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发表时间：
2017-01-31
影响因子：
4.6
通讯作者：
Kurumizaka H
中科院分区：
文献类型：
--
作者：
Osakabe A;Arimura Y;Matsumoto S;Horikoshi N;Sugasawa K;Kurumizaka H
基因组中产生大量（> 10，000/天）脱嘌呤/脱嘧啶（AP）位点，但它们在染色质中的结构仍不确定。我们确定了在两个对称位点含有AP位点类似物的核小体的晶体结构，这揭示了结构多态性：一个在AP位点形成没有空位的尺蠖构型，另一个形成具有空位和孤儿碱基的B型样结构。AP位点的这种意想不到的尺蠖构型对于了解AP DNA修复机制非常重要，因为在碱基切除修复过程中，它可能不会被主要的AP结合蛋白APE 1识别。
Huge amounts (>10,000/day) of apurinic/apyrimidinic (AP) sites are produced in genomes, but their structures in chromatin remain undetermined. We determined the crystal structure of the nucleosome containing AP-site analogs at two symmetric sites, which revealed structural polymorphism: one forms an inchworm configuration without an empty space at the AP site, and the other forms a B-form-like structure with an empty space and the orphan base. This unexpected inchworm configuration of the AP site is important to understand the AP DNA repair mechanism, because it may not be recognized by the major AP-binding protein, APE1, during the base excision repair process.